Chen J, Zhou Q, Qin Y, Sun Z, Sun Z, Wang Y, Li L, Qin J
Department of Thor acocardiac Surgery, The First Univer sity Hospital , West China University of Medical Sciences, Chengdu, Sichuan 610041, P. R. China.
Zhongguo Fei Ai Za Zhi. 2000 Aug 20;3(4):245-9. doi: 10.3779/j.issn.1009-3419.2000.04.02.
To explore the role of abnormalities in RNA transcript of FHIT gene in human non-small cell lung cancer(NSCLC).
Transcript abnormalities of FHIT gene were detected in 35 cancer samples and their corresponding paracancer tissues and normal tissues, four lung cancer cell lines, and 10 lung tissues of benign pulmonary lesions as control by nested RT-PCR.
Fourteen of 35 cancer tissues (40%) had abnormalities in RNA transcripts of FHIT. There were 5 paracancer tissues with transcript abnormalities of FHIT gene out of the 14 lung cancers with transcript abnormalities of FHIT gene, with an abnormality rate of 35.71%. All of 4 lung cancer cell lines had transcript abnormalities of FHIT gene. The aberrant rate of FHIT gene in squamous cell carcinoma tissues (58.82%) was significantly higher than that in adenocarcinoma tissues (22.22%) (P<0.05). No relationship was found among the transcript abnormality of the FHIT gene and differentiated degree of the tumor cell, stages of the cancer, size of the primary tumor, location of cancer, and age of the patients (P>0.05).
The results show that there are transcript abnormalities of FHIT gene in NSCLC. FHIT transcript abnormality preferentially occur in squamous cell carcinomas, and it might be the early molecular phenomenon of lung cancer.
探讨脆性组氨酸三联体（FHIT）基因RNA转录本异常在人类非小细胞肺癌（NSCLC）中的作用。
采用巢式逆转录聚合酶链反应（nested RT-PCR）检测35例癌组织及其相应癌旁组织和正常组织、4种肺癌细胞系以及10例肺部良性病变组织中的FHIT基因转录本异常情况。
35例癌组织中有14例（40%）FHIT基因RNA转录本存在异常。在14例FHIT基因转录本异常的肺癌中，有5例癌旁组织存在FHIT基因转录本异常，异常率为35.71%。4种肺癌细胞系均存在FHIT基因转录本异常。鳞状细胞癌组织中FHIT基因异常率（58.82%）显著高于腺癌组织（22.22%）（P<0.05）。未发现FHIT基因转录本异常与肿瘤细胞分化程度、癌症分期、原发肿瘤大小、癌症部位及患者年龄之间存在相关性（P>0.05）。
结果表明NSCLC中存在FHIT基因转录本异常。FHIT转录本异常在鳞状细胞癌中更易发生，可能是肺癌的早期分子现象。
