Egberts J, Sietaram M A
Department of Obstetrics and Gynecology, Leiden University Medical Center, The Netherlands.
Respiration. 1990;57(4):254-8. doi: 10.1159/000195851.
Isolation of alveolar surfactant from human cadaver lungs might be ineffective because of postmortem effects. We studied therefore in the rat the effect of autolysis on the yield and composition of alveolar surfactant at different intervals after death. The total amount of phospholipids in the lavage fluids decreased at 4 h postmortem and increased thereafter again. Increased amounts of proteins, significant deviations from the normal phospholipid composition of surfactant and decreased surface activity were already present from 2 h onwards. However, a normal alveolar surfactant can be obtained up to 16 h after death by using a sucrose gradient centrifugation procedure. With this procedure it is possible to isolate a surfactant with adequate surface activity from mildly, but not from severely autolytic rat lungs.
由于死后效应，从人类尸体肺中分离肺泡表面活性物质可能无效。因此，我们在大鼠中研究了死后不同时间间隔自溶对肺泡表面活性物质产量和成分的影响。死后4小时灌洗液中磷脂总量减少，之后又再次增加。从死后2小时起，蛋白质含量增加、表面活性物质的正常磷脂组成出现显著偏差以及表面活性降低。然而，通过使用蔗糖梯度离心法，在死后长达16小时仍可获得正常的肺泡表面活性物质。通过该方法，有可能从轻度自溶但非重度自溶的大鼠肺中分离出具有足够表面活性的表面活性物质。
