Department of Cancer and Inflammation Research, University of Southern Denmark, Odense, Denmark.
J Immunol. 2010 Nov 15;185(10):6096-104. doi: 10.4049/jimmunol.1002185. Epub 2010 Oct 18.
Collectins play important roles in the innate immune defense against microorganisms. Recently, a new collectin, collectin 11 (CL-11 or CL-K1), was identified via database searches. In present work, we characterize the structural and functional properties of CL-11. Under nonreducing conditions, in gel permeation chromatography recombinant CL-11 forms disulfide-linked oligomers of 100 and 200 kDa. A mAb-based ELISA estimates the concentration of CL-11 in plasma to be 2.1 μg/ml, and the presence of CL-11 in plasma was further verified by Western blotting and mass spectrometry. Mannan-binding lectin-associated serine protease 1 (MASP-1) copurified with CL-11 and the interaction in plasma with MASP-1 and/or MASP-3 was further demonstrated using ELISA. We identified the adrenal glands, the kidneys, and the liver as primary sites of expression. CL-11 lectin activity was demonstrated by ELISA and showed that CL-11 has preference for l-fucose and d-mannose. We finally show that CL-11 binds to intact bacteria, fungi, and viruses and that CL-11 decreases influenza A virus infectivity and forms complexes with DNA. On the basis of the significant concentration of CL-11 in circulation and CL-11's interaction with various microorganisms and MASP-1 and/or MASP-3, it is conceivable that CL-11 plays a role in activation of the complement system and in the defense against invading microorganisms.
收集素在先天免疫防御微生物中起着重要作用。最近，通过数据库搜索鉴定了一种新的收集素，即收集素 11（CL-11 或 CL-K1）。在本工作中，我们描述了 CL-11 的结构和功能特性。在非还原条件下，在凝胶渗透色谱中，重组 CL-11 形成 100 和 200 kDa 的二硫键连接的寡聚物。基于 mAb 的 ELISA 估计 CL-11 在血浆中的浓度为 2.1 μg/ml，并且通过 Western blot 和质谱法进一步证实了 CL-11 在血浆中的存在。甘露聚糖结合凝集素相关丝氨酸蛋白酶 1（MASP-1）与 CL-11 共纯化，并且在血浆中与 MASP-1 和/或 MASP-3 的相互作用进一步通过 ELISA 证明。我们鉴定了肾上腺、肾脏和肝脏为主要表达部位。通过 ELISA 证明了 CL-11 的凝集素活性，并且表明 CL-11 对 l-岩藻糖和 d-甘露糖具有偏好性。我们最后表明 CL-11 结合完整的细菌、真菌和病毒，并且 CL-11 降低流感 A 病毒感染性并与 DNA 形成复合物。鉴于 CL-11 在循环中的显著浓度以及 CL-11 与各种微生物和 MASP-1 和/或 MASP-3 的相互作用，可以想象 CL-11 在补体系统的激活和抵御入侵微生物方面发挥作用。
