Zhu Gen-ying, Zhou Mou-wang, Huang Hong-shi, Chen Ya-ping, Wang Wen-ting, Zeng Fan-shuo
Department of Rehabilitation Medicine, Peking University Third Hospital, Beijing 100191, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2010 Oct 18;42(5):530-4.
To study the highly selective dorsal rhizotomy influence on bladder and penis erection function of SD rats.
Forty mature male SD rats of clean grades, with weight range of 300-350 g were selected. Ten rats were chosen to do electrophysiology study on dorsal rootlets of L6 and S1 segment. Changes in intravesical pressure (IVP) and intracavernous pressure (ICP) were investigated to define main segments which conducted to bladder and corpus cavernosum. Thirty rats were divided into two groups (A and B) on average at random. In group A, we made highly selective dorsal rhizotomy on the fascicle of conduction bladder detrusor muscle. In group B, we made highly selective dorsal rhizotomy on the fascicle of conduction corpus cavernosum. Changes of IVP and ICP after rhizotomy were investigated and recorded.
The changes of IVP during electrostimulation were of no significant variation between L6 and S1 (P=0.972). With the changes of ICP during electrostimulation, S1 segment was of more significant variation than L6 segment, ΔICP of S1 was (13.05±8.41) cmH2O (1 cmH2O=0.098 kPa), while ΔICP of L6 was (6.88±2.76) cmH2O (P<0.01). There was no reasonable variation in IVP and ICP on the left and right dorsal rootlets of S1 segment (P was 0.623 and 0.828 respectively). In group A, there was significant variation in IVP, ΔIVP of before rhizotomy was (14.37±4.89) cmH2O, while after rhizotomy was (3.25±1.29) cmH2O (P<0.001) while no obvious variation in ICP (P=0.153) after highly selective rhizotomy on S1 dorsal rootlets. In group B, there was significant variation in ICP, ΔICP of before rhizotomy was (11.97±4.41) cmH2O, while after rhizotomy was (2.68±1.01) cmH2O (P<0.001), but no obvious variation in IVP (P=0.162) after highly selective rhizotomy on S1 dorsal rootlets.
SD rats' different rootlets of S1 dorsal rootlets can be distinguished by microanatomy and electrostimulation. The IVP and ICP had distinct changes after highly selective dorsal rhizotomy. It could provide an experimental support to treat spastic bladder after spinal cord injury and retain at maximum reflexible erection function in the clinic.
研究高选择性脊神经后根切断术对SD大鼠膀胱及阴茎勃起功能的影响。
选取40只清洁级成年雄性SD大鼠，体重300 - 350 g。选取10只大鼠进行L6和S1节段背根小束的电生理研究。通过观察膀胱内压（IVP）和海绵体内压（ICP）的变化来确定传导至膀胱和海绵体的主要节段。将30只大鼠随机平均分为两组（A组和B组）。A组在传导膀胱逼尿肌的神经束上进行高选择性脊神经后根切断术。B组在传导海绵体的神经束上进行高选择性脊神经后根切断术。观察并记录脊神经后根切断术后IVP和ICP的变化。
电刺激时L6和S1节段IVP的变化无显著差异（P = 0.972）。电刺激时ICP的变化，S1节段比L6节段更显著，S1节段的ΔICP为（13.05±8.41）cmH₂O（1 cmH₂O = 0.098 kPa），而L6节段的ΔICP为（6.88±2.76）cmH₂O（P < 0.01）。S1节段左右背根小束的IVP和ICP无合理差异（P分别为0.623和0.828）。A组中，IVP有显著变化，脊神经后根切断术前的ΔIVP为（14.37±4.89）cmH₂O，而术后为（3.25±1.29）cmH₂O（P < 0.001），而在S1背根小束进行高选择性脊神经后根切断术后ICP无明显变化（P = 0.153）。B组中，ICP有显著变化，脊神经后根切断术前的ΔICP为（11.97±4.41）cmH₂O，而术后为（2.68±1.01）cmH₂O（P < 0.001），但在S1背根小束进行高选择性脊神经后根切断术后IVP无明显变化（P = 0.162）。
通过显微解剖和电刺激可区分SD大鼠S1背根的不同小束。高选择性脊神经后根切断术后IVP和ICP有明显变化。可为临床治疗脊髓损伤后痉挛性膀胱并最大程度保留反射性勃起功能提供实验依据。
