预测蛋白转位马达蛋白中的新型多脂结合区：一种可能的普遍特征。
The prediction of novel multiple lipid-binding regions in protein translocation motor proteins: a possible general feature.
机构信息
Section Chemistry, Charlemagne College, Wilhelminastraat 13-15, Nijmegen, 3913 NH, The Netherlands.
出版信息
Cell Mol Biol Lett. 2011 Mar;16(1):40-54. doi: 10.2478/s11658-010-0036-y. Epub 2010 Oct 19.
Abstract
Protein translocation is an important cellular process. SecA is an essential protein component in the Sec system, as it contains the molecular motor that facilitates protein translocation. In this study, a bioinformatics approach was applied in the search for possible lipid-binding helix regions in protein translocation motor proteins. Novel lipid-binding regions in Escherichia coli SecA were identified. Remarkably, multiple lipid-binding sites were also identified in other motor proteins such as BiP, which is involved in ER protein translocation. The prokaryotic signal recognition particle receptor FtsY, though not a motor protein, is in many ways related to SecA, and was therefore included in this study. The results demonstrate a possible general feature for motor proteins involved in protein translocation.
摘要
蛋白质易位是一种重要的细胞过程。SecA 是 Sec 系统中的一种必需蛋白成分，因为它包含促进蛋白质易位的分子马达。在这项研究中，应用了一种生物信息学方法来寻找蛋白质易位马达蛋白中可能的脂质结合螺旋区域。在大肠杆菌 SecA 中发现了新的脂质结合区域。值得注意的是，还在其他马达蛋白中鉴定到了多个脂质结合位点，例如参与 ER 蛋白易位的 BiP。尽管原核信号识别颗粒受体 FtsY 不是一种马达蛋白，但在许多方面与 SecA 相关，因此也包括在本研究中。结果表明，参与蛋白质易位的马达蛋白具有一个可能的普遍特征。
相似文献
1
The prediction of novel multiple lipid-binding regions in protein translocation motor proteins: a possible general feature.预测蛋白转位马达蛋白中的新型多脂结合区：一种可能的普遍特征。
Cell Mol Biol Lett. 2011 Mar;16(1):40-54. doi: 10.2478/s11658-010-0036-y. Epub 2010 Oct 19.
2
Oligomeric states of the SecA and SecYEG core components of the bacterial Sec translocon.细菌Sec转运体SecA和SecYEG核心组分的寡聚状态。
Biochim Biophys Acta. 2007 Jan;1768(1):5-12. doi: 10.1016/j.bbamem.2006.08.013. Epub 2006 Aug 30.
3
The dispensability and requirement of SecA N-terminal aminoacyl residues for complementation, membrane binding, lipid-specific domains and channel activities.SecA N 端氨酰基残基在互补、膜结合、脂质特异性结构域和通道活性方面的可缺失性及必要性。
Biochem Biophys Res Commun. 2014 Oct 10;453(1):138-42. doi: 10.1016/j.bbrc.2014.09.080. Epub 2014 Sep 27.
4
The bacterial SRP receptor, SecA and the ribosome use overlapping binding sites on the SecY translocon.细菌 SRP 受体、SecA 和核糖体在 SecY 转运蛋白上使用重叠的结合位点。
Traffic. 2011 May;12(5):563-78. doi: 10.1111/j.1600-0854.2011.01167.x. Epub 2011 Feb 25.
5
SecA, the motor of the secretion machine, binds diverse partners on one interactive surface.SecA作为分泌机器的动力蛋白，在一个相互作用表面上结合多种伙伴。
J Mol Biol. 2008 Sep 26;382(1):74-87. doi: 10.1016/j.jmb.2008.06.049. Epub 2008 Jun 24.
6
Structural basis for signal-sequence recognition by the translocase motor SecA as determined by NMR.通过核磁共振确定的转运体马达SecA对信号序列识别的结构基础。
Cell. 2007 Nov 16;131(4):756-69. doi: 10.1016/j.cell.2007.09.039.
7
Dimeric SecA couples the preprotein translocation in an asymmetric manner.二聚体 SecA 以不对称的方式偶联前蛋白的易位。
PLoS One. 2011 Jan 27;6(1):e16498. doi: 10.1371/journal.pone.0016498.
8
The long alpha-helix of SecA is important for the ATPase coupling of translocation.SecA的长α螺旋对于转运过程中的ATP酶偶联很重要。
J Biol Chem. 2006 Nov 24;281(47):36249-56. doi: 10.1074/jbc.M606906200. Epub 2006 Sep 27.
9
SecA interacts with ribosomes in order to facilitate posttranslational translocation in bacteria.SecA 与核糖体相互作用，以促进细菌中的翻译后易位。
Mol Cell. 2011 Feb 4;41(3):343-53. doi: 10.1016/j.molcel.2010.12.028.
10
Orientation of SecA and SecB in complex, derived from disulfide cross-linking.SecA 和 SecB 复合物的取向，源自二硫键交联。
J Bacteriol. 2011 Jan;193(1):190-6. doi: 10.1128/JB.00975-10. Epub 2010 Oct 29.
引用本文的文献
1
Topology of the SecA ATPase Bound to Large Unilamellar Vesicles.SecA ATPase 与大单室囊泡的拓扑结构。
J Mol Biol. 2022 Jun 30;434(12):167607. doi: 10.1016/j.jmb.2022.167607. Epub 2022 Apr 27.
2
identification of lipid-binding α helices of uncoupling protein 1.解偶联蛋白1脂质结合α螺旋的鉴定
Biomed Rep. 2018 Oct;9(4):313-317. doi: 10.3892/br.2018.1133. Epub 2018 Jul 23.
3
SecA inhibitors as potential antimicrobial agents: differential actions on SecA-only and SecA-SecYEG protein-conducting channels.作为潜在抗菌剂的SecA抑制剂：对仅含SecA和SecA-SecYEG蛋白质传导通道的不同作用。
FEMS Microbiol Lett. 2018 Aug 1;365(15). doi: 10.1093/femsle/fny145.
4
Penetration into membrane of amino-terminal region of SecA when associated with SecYEG in active complexes.与 SecYEG 结合在活性复合物中时，SecA 的氨基末端区域穿透膜。
Protein Sci. 2018 Mar;27(3):681-691. doi: 10.1002/pro.3362. Epub 2018 Feb 5.
5
Dissecting structures and functions of SecA-only protein-conducting channels: ATPase, pore structure, ion channel activity, protein translocation, and interaction with SecYEG/SecDF•YajC.仅含SecA的蛋白质传导通道的结构与功能剖析：ATP酶、孔结构、离子通道活性、蛋白质转运以及与SecYEG/SecDF•YajC的相互作用
PLoS One. 2017 Jun 2;12(6):e0178307. doi: 10.1371/journal.pone.0178307. eCollection 2017.
6
The role and significance of potential lipid-binding regions in the mitochondrial protein import motor: an in-depth in silico study.线粒体蛋白质输入马达中潜在脂质结合区域的作用及意义：一项深入的计算机模拟研究
3 Biotech. 2015 Dec;5(6):1041-1051. doi: 10.1007/s13205-015-0310-9. Epub 2015 May 23.
7
Highly potent antimicrobial peptides from N-terminal membrane-binding region of E. coli MreB.来自大肠杆菌 MreB N 端膜结合区的高效抗菌肽。
Sci Rep. 2017 Feb 23;7:42994. doi: 10.1038/srep42994.
8
Identification and in silico analysis of helical lipid binding regions in proteins belonging to the amphitropic protein family.两亲性蛋白家族成员中螺旋状脂质结合区域的鉴定及计算机模拟分析
J Biosci. 2014 Dec;39(5):771-83. doi: 10.1007/s12038-014-9479-z.
9
The dispensability and requirement of SecA N-terminal aminoacyl residues for complementation, membrane binding, lipid-specific domains and channel activities.SecA N 端氨酰基残基在互补、膜结合、脂质特异性结构域和通道活性方面的可缺失性及必要性。
Biochem Biophys Res Commun. 2014 Oct 10;453(1):138-42. doi: 10.1016/j.bbrc.2014.09.080. Epub 2014 Sep 27.
10
Prediction of lipid-binding regions in cytoplasmic and extracellular loops of membrane proteins as exemplified by protein translocation membrane proteins.以蛋白转位膜蛋白为例预测细胞质和细胞外环的膜蛋白中的脂质结合区域。
J Membr Biol. 2013 Jan;246(1):21-9. doi: 10.1007/s00232-012-9498-3. Epub 2012 Sep 9.
本文引用的文献
1
Linking new paradigms in protein chemistry to reversible membrane-protein interactions.将蛋白质化学中的新范式与可逆膜蛋白相互作用联系起来。
Curr Protein Pept Sci. 2009 Aug;10(4):339-59. doi: 10.2174/138920309788922199.
2
Two cooperating helices constitute the lipid-binding domain of the bacterial SRP receptor.两条相互协作的螺旋构成了细菌信号识别颗粒（SRP）受体的脂质结合结构域。
J Mol Biol. 2009 Jul 17;390(3):401-13. doi: 10.1016/j.jmb.2009.04.061. Epub 2009 May 3.
3
HELIQUEST: a web server to screen sequences with specific alpha-helical properties.HELIQUEST：一个用于筛选具有特定α螺旋特性序列的网络服务器。
Bioinformatics. 2008 Sep 15;24(18):2101-2. doi: 10.1093/bioinformatics/btn392. Epub 2008 Jul 28.
4
SecA, the motor of the secretion machine, binds diverse partners on one interactive surface.SecA作为分泌机器的动力蛋白，在一个相互作用表面上结合多种伙伴。
J Mol Biol. 2008 Sep 26;382(1):74-87. doi: 10.1016/j.jmb.2008.06.049. Epub 2008 Jun 24.
5
A cleavable N-terminal membrane anchor is involved in membrane binding of the Escherichia coli SRP receptor.一种可裂解的N端膜锚定蛋白参与大肠杆菌信号识别颗粒受体的膜结合。
J Mol Biol. 2008 Mar 28;377(3):761-73. doi: 10.1016/j.jmb.2008.01.040. Epub 2008 Jan 26.
6
Membrane phosphatidylserine regulates surface charge and protein localization.膜磷脂酰丝氨酸调节表面电荷和蛋白质定位。
Science. 2008 Jan 11;319(5860):210-3. doi: 10.1126/science.1152066.
7
Protein translocation across the bacterial cytoplasmic membrane.蛋白质跨细菌细胞质膜的转运。
Annu Rev Biochem. 2008;77:643-67. doi: 10.1146/annurev.biochem.77.061606.160747.
8
Escherichia coli signal recognition particle receptor FtsY contains an essential and autonomous membrane-binding amphipathic helix.大肠杆菌信号识别颗粒受体FtsY包含一个必需的、自主的膜结合两亲性螺旋。
J Biol Chem. 2007 Nov 2;282(44):32176-84. doi: 10.1074/jbc.M705430200. Epub 2007 Aug 28.
9
To be or not to be: predicting soluble SecAs as membrane proteins.存在还是不存在：预测作为膜蛋白的可溶性SecA蛋白
IEEE Trans Nanobioscience. 2007 Jun;6(2):168-79. doi: 10.1109/tnb.2007.897486.
10
Pushing, pulling and trapping--modes of motor protein supported protein translocation.推动、拉动和捕获——马达蛋白支持的蛋白质转运模式。
FEBS Lett. 2007 Jun 19;581(15):2820-8. doi: 10.1016/j.febslet.2007.04.015. Epub 2007 Apr 18.
Zotero 插件