Department of Sports Medicine, College of Health Care, China Medical University and University Hospital, Taichung, Taiwan, ROC.
J Orthop Res. 2011 Apr;29(4):495-500. doi: 10.1002/jor.21261. Epub 2010 Oct 18.
Progression to osteoarthritis (OA) is a frequent sequela of severe articular fracture, particularly when weight-bearing joints are involved. Prevention from post-traumatic OA remains a challenge. Hyaluronan (HA) therapy is reported to represent a safe and effective treatment for patients with OA and rheumatoid arthritis. However, the capacity of HA to prevent the occurrence of osteoarthritic changes in fractured joints has not been demonstrated. The present study was undertaken to examine the effects of HA on expression of six OA-related proteins in fibroblast-like synoviocytes (FLS) from 10 patients with tibia plateau fracture. OA-related factors were quantified using a sandwich enzyme-linked immunosorbent assay. Regardless of induction of the FLS with interleukin (IL)-1β, HA was found to down-regulate expression of catabolic factors (IL-1β, matrix metalloproteinase-3, and tumor necrosis factor-α) and to up-regulate production of anti-catabolic factors (tissue inhibitors of metalloproteinase-1 and metalloproteinase-2). HA also enhanced expression of IL-10, an anti-inflammatory cytokine, in FLS. Our results indicated that HA can promote the expression of both antiinflammatory and structure-protective factors in FLS of patients with tibia plateau fracture.
骨关节炎（OA）的进展是严重关节骨折的常见后遗症，尤其是当负重关节受累时。预防创伤后 OA 仍然是一个挑战。透明质酸（HA）治疗被报道对 OA 和类风湿关节炎患者是一种安全有效的治疗方法。然而，HA 预防骨折关节发生骨关节炎变化的能力尚未得到证实。本研究旨在研究 HA 对 10 例胫骨平台骨折患者成纤维样滑膜细胞（FLS）中 6 种 OA 相关蛋白表达的影响。使用夹心酶联免疫吸附试验定量测定 OA 相关因素。无论是否用白细胞介素（IL）-1β诱导 FLS，HA 均下调分解代谢因子（IL-1β、基质金属蛋白酶-3 和肿瘤坏死因子-α）的表达，并上调抗分解代谢因子（组织抑制剂的表达-1 和基质金属蛋白酶-2）。HA 还增强了 FLS 中抗炎细胞因子白细胞介素-10 的表达。我们的结果表明，HA 可以促进胫骨平台骨折患者 FLS 中抗炎和结构保护因子的表达。
