Brunner A, de Boland A R
Departamento de Biologia, Universidad Nacional del Sur, Bahia Blanca, Argentina.
Z Naturforsch C J Biosci. 1990 Nov-Dec;45(11-12):1156-60. doi: 10.1515/znc-1990-11-1212.
Incubation of chick embryo myoblasts with 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] (10(-10) M, 24 h), markedly stimulated the incorporation of [3H]leucine into total cytoskeletal proteins and this effect was abolished when the sterol treatment was performed in the presence of cycloheximide or actinomycin D. 1,25(OH)2D3 selectively stimulated the de novo synthesis of several proteins with apparent molecular masses (isoelectric points) of 220 kDa (6.1 and 9.7), 150 kDa (7.5), 110 kDa (7.2), 68 kDa (9.5, 7.5 and 4.5), 50 kDa (8.5), 44 kDa (6.3), 27 kDa (7.8) and 15 kDa (5.5). Labelling of proteins with [125I]calmodulin after their separation on SDS-polyacrylamide gels showed that 1,25(OH)2D3-dependent protein of 110 kDa is the major calmodulin-binding component of myoblasts cytoskeleton. In addition, the sterol increased the phosphorylation of several cytoskeletal proteins including that of 110 and 15 kDa whose synthesis potentiates.
用1,25 - 二羟维生素D3 [1,25(OH)2D3]（10^(-10) M，24小时）孵育鸡胚成肌细胞，显著刺激了[3H]亮氨酸掺入总细胞骨架蛋白中，并且当在放线菌酮或放线菌素D存在的情况下进行固醇处理时，这种效应被消除。1,25(OH)2D3选择性地刺激了几种蛋白质的从头合成，这些蛋白质的表观分子量（等电点）分别为220 kDa（6.1和9.7）、150 kDa（7.5）、110 kDa（7.2）、68 kDa（9.5、7.5和4.5）、50 kDa（8.5）、44 kDa（6.3）、27 kDa（7.8）和15 kDa（5.5）。在SDS - 聚丙烯酰胺凝胶上分离后用[125I]钙调蛋白标记蛋白质表明，110 kDa的1,25(OH)2D3依赖性蛋白是成肌细胞细胞骨架的主要钙调蛋白结合成分。此外，固醇增加了几种细胞骨架蛋白的磷酸化，包括合成增强的110 kDa和15 kDa蛋白的磷酸化。
