钠离子-钾离子-ATP 酶活性和亚基蛋白表达：羊大脑皮质和肾皮质的个体发生以及外源性和内源性类固醇的影响。
Na+,K+-ATPase activity and subunit protein expression: ontogeny and effects of exogenous and endogenous steroids on the cerebral cortex and renal cortex of sheep.
机构信息
Department of Pediatrics, Women and Infants Hospital of Rhode Island, The Warren Alpert Medical School of Brown University, Providence, RI 02905,USA.
出版信息
Reprod Sci. 2011 Apr;18(4):359-73. doi: 10.1177/1933719110385137. Epub 2010 Oct 19.
We examined the effects of development, exogenous, and endogenous glucocorticoids on Na(+),K(+)-ATPase activity and subunit protein expression in ovine cerebral cortices and renal cortices. Ewes at 60%, 80%, and 90% gestation, newborns, and adults received 4 dexamethasone or placebo injections. Cerebral cortex Na(+),K(+)-ATPase activity was higher (P < .05) in placebo-treated newborns than fetuses of placebo-treated ewes and adults, α(1)-expression was higher at 90% gestation than the other ages; α(2)-expression was higher in newborns than fetuses; α(3)-expression was higher in newborns than 60% gestation; β(1)-expression was higher in newborns than the other ages, and β(2)-expression higher at 60% than 80% and 90% gestation, and in adults. Renal cortex Na(+),K(+)-ATPase activity was higher in placebo-treated adults and newborns than fetuses. Cerebral cortex Na(+),K(+)-ATPase activity was higher in dexamethasone- than placebo-treated adults, and α(1)-expression higher in fetuses of dexamethasone- than placebo-treated ewes at 60% and 80% gestation. Renal cortex Na(+),K(+)-ATPase activity and α(1)-expression were higher in fetuses of dexamethasone- than placebo-treated ewes at each gestational age, and β(1)-expression was higher in fetuses of dexamethasone- than placebo-treated ewes at 90% gestation and in dexamethasone- than placebo-treated adults. Cerebral cortex Na(+),K(+)-ATPase activity, α(1)-expression, β(1)-expression, and renal cortex α(1)-expression correlated directly with increases in fetal cortisol. In conclusion, Na(+),K(+)-ATPase activity and subunit expression exhibit specific developmental patterns in brain and kidney; exogenous glucocorticoids regulate activity and subunit expression in brain and kidney at some ages; endogenous increases in fetal cortisol regulate cerebral Na(+),K(+)-ATPase, but exogenous glucocorticoids have a greater effect on renal than cerebral Na(+),K(+)-ATPase.
我们研究了发育、外源性和内源性糖皮质激素对绵羊大脑皮质和肾脏皮质 Na(+),K(+)-ATP 酶活性和亚基蛋白表达的影响。60%、80%和 90%孕期、新生儿和成年母羊接受 4 次地塞米松或安慰剂注射。皮质 Na(+),K(+)-ATP 酶活性在接受安慰剂的新生儿中较高（P<.05），而接受安慰剂的胎儿比成年母羊低；α(1)-表达在 90%孕期高于其他年龄；α(2)-表达在新生儿中高于胎儿；α(3)-表达在新生儿中高于 60%孕期；β(1)-表达在新生儿中高于其他年龄，β(2)-表达在 60%孕期高于 80%和 90%孕期，以及成年期。肾皮质 Na(+),K(+)-ATP 酶活性在接受安慰剂的成年母羊和新生儿中高于胎儿。皮质 Na(+),K(+)-ATP 酶活性在接受地塞米松的成年母羊中高于接受安慰剂的成年母羊，α(1)-表达在接受地塞米松的胎儿中高于接受安慰剂的 60%和 80%孕期的母羊。肾皮质 Na(+),K(+)-ATP 酶活性和 α(1)-表达在每个孕期接受地塞米松的胎儿中均高于接受安慰剂的母羊，β(1)-表达在接受地塞米松的胎儿中高于接受安慰剂的母羊在 90%孕期和接受地塞米松的成年母羊中。皮质 Na(+),K(+)-ATP 酶活性、α(1)-表达、β(1)-表达和肾皮质 α(1)-表达与胎儿皮质醇的增加呈正相关。总之，Na(+),K(+)-ATP 酶活性和亚基表达在大脑和肾脏中表现出特定的发育模式；外源性糖皮质激素在某些年龄调节大脑和肾脏的活性和亚基表达；胎儿皮质醇的内源性增加调节大脑 Na(+),K(+)-ATP 酶，但外源性糖皮质激素对肾脏 Na(+),K(+)-ATP 酶的影响大于大脑。
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