Department of Membrane Transport, Institute of Physiology, Academy of Sciences of the Czech Republic, v.v.i, Videnska 1083, 14220 Prague 4, Czech Republic.
Fungal Biol. 2010 Feb-Mar;114(2-3):144-50. doi: 10.1016/j.funbio.2009.11.002.
Saccharomyces cerevisiae yeast cells serve as a model to elucidate the bases of salt tolerance and potassium homeostasis regulation in eukaryotic cells. In this study, we show that two widely used laboratory strains, BY4741 and W303-1A, differ not only in cell size and volume but also in their relative plasma-membrane potential (estimated with a potentiometric fluorescent dye diS-C3(3) and as Hygromycin B sensitivity) and tolerance to alkali-metal cations. W303-1A cells and their mutant derivatives lacking either uptake (trk1 trk2) or efflux (nha1) systems for alkali-metal cations are more tolerant to toxic sodium and lithium cations but also more sensitive to higher external concentrations of potassium than BY4741 cells and their mutants. Moreover, our results suggest that though the two strains do not differ in the total potassium content, the regulation of intracellular potassium homeostasis is probably not the same in BY4741 and W303-1A cells.
酿酒酵母细胞可用作模型来阐明真核细胞耐盐性和钾离子稳态调节的基础。在这项研究中，我们表明，两种广泛使用的实验室菌株 BY4741 和 W303-1A，不仅在细胞大小和体积上有所不同，而且在相对质膜电位（用荧光染料 diS-C3(3) 并根据 Hygromycin B 敏感性来估计）以及对碱金属阳离子的耐受性方面也有所不同。W303-1A 细胞及其突变体衍生物，无论是缺失用于摄取（trk1 trk2）还是外排（nha1）碱金属阳离子的系统，对毒性钠离子和锂离子的耐受性更强，但对更高浓度的钾离子也更敏感，而 BY4741 细胞及其突变体则不是这样。此外，我们的结果表明，尽管这两种菌株的总钾含量没有差异，但 BY4741 和 W303-1A 细胞中细胞内钾离子稳态的调节可能并不相同。
