蛋白质亚磺酸含量的测定。
Measurement of protein sulfenic acid content.
作者信息
Poole Leslie B
机构信息
Wake Forest University School of Medicine, Winston-Salem, North Carolina.
出版信息
Curr Protoc Toxicol. 2008 Nov;Chapter 17:Unit17.2. doi: 10.1002/0471140856.tx1702s38.
Abstract
Protein sulfenic acids are reactive, reversibly oxidized cysteinyl residues with roles in redox catalysis and regulation. Detection and quantification of these species in proteins is accomplished through chemical modification by reagents such as 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD chloride), 2-nitro-5-thiobenzoate (TNB), dimedone, or derivatives of dimedone, followed by UV-visible spectroscopy or mass spectrometric analysis.
摘要
蛋白质亚磺酸是具有反应活性的、可逆氧化的半胱氨酰残基，在氧化还原催化和调节中发挥作用。蛋白质中这些物质的检测和定量是通过用7-氯-4-硝基苯并-2-恶唑-1,3-二唑（NBD氯化物）、2-硝基-5-硫代苯甲酸（TNB）、双甲酮或双甲酮衍生物等试剂进行化学修饰，然后进行紫外-可见光谱或质谱分析来完成的。
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