Cepeda-Valdés R, Pohla-Gubo G, Borbolla-Escoboza J R, Barboza-Quintana O, Ancer-Rodríguez J, Hintner H, Salas-Alanis J C
Centro de Investigación Clínica, InstitutoTecnológico y de Estudios Superiores de Monterrey, México.
Actas Dermosifiliogr. 2010 Oct;101(8):673-82.
The tools for diagnosis of epidermolysis bullosa have advanced greatly since Hintner's group introduced antigen mapping as a diagnostic test for this family of genodermatoses. Monoclonal or polyclonal antibodies raised against some of the specific proteins found in the epidermis and basement membrane of the epidermis have allowed 4 types of epidermolysis bullosa de be identified and all variants to be classified. When a newborn baby presents with blisters, many conditions are implicated in the differential diagnosis. Examination under an optical microscope can suggest epidermolysis bullosa, but immunofluorescence mapping and electron microscopy are required for confirmation of the diagnosis and further classification of congenital epidermolysis bullosa. This article explains the importance of immunofluorescence antigen mapping and describes the methods employed for classification and subclassification of epidermolysis bullosa.
自从欣特纳团队将抗原图谱作为这类遗传性皮肤病的诊断测试引入以来，大疱性表皮松解症的诊断工具已经有了很大进展。针对在表皮和表皮基底膜中发现的一些特定蛋白质产生的单克隆或多克隆抗体，使得能够识别4种类型的大疱性表皮松解症，并对所有变体进行分类。当新生儿出现水疱时，鉴别诊断涉及多种情况。光学显微镜检查可提示大疱性表皮松解症，但确诊以及先天性大疱性表皮松解症的进一步分类需要免疫荧光图谱和电子显微镜检查。本文解释了免疫荧光抗原图谱的重要性，并描述了用于大疱性表皮松解症分类和亚分类的方法。
