Department of Biology, Faculty of Science, Burapha University, Chon Buri 20131, Thailand.
J Invertebr Pathol. 2011 Feb;106(2):236-41. doi: 10.1016/j.jip.2010.10.003. Epub 2010 Oct 20.
Adult workers of Apis cerana, Apis florea and Apis mellifera from colonies heavily infected with Nosema ceranae were selected for molecular analyses of the parasite. PCR-specific 16S rRNA primers were designed, cloned, sequenced and compared to GenBank entries. The sequenced products corresponded to N. ceranae. We then infected A. cerana with N. ceranae spores isolated from A. florea workers. Newly emerged bees from healthy colonies were fed 10,000, 20,000 and 40,000 spores/bee. There were significant dosage dependent differences in bee infection and survival rates. The ratio of infected cells to non-infected cells increased at 6, 10 and 14 d post infection. In addition, hypopharyngeal glands of bees from the control group had significantly higher protein concentrations than infected groups. Bees infected with 40,000 spores/bee had the lowest protein concentrations. Thus, N. ceranae isolated from A. florea is capable of infecting another bee species, impairing hypopharyngeal gland protein production and reducing bee survival in A. cerana.
从感染严重的中华蜜蜂、东方蜜蜂和意大利蜜蜂群体中选择成年工蜂，进行寄生虫的分子分析。设计、克隆、测序并比较了针对寄生虫 16S rRNA 的 PCR 特异性引物，并与 GenBank 条目进行了比较。测序产物与被囊菌属 N. ceranae 相对应。然后，我们用从东方蜜蜂工蜂中分离出的被囊菌属 N. ceranae 孢子感染中华蜜蜂。从健康群体中孵化的新蜜蜂被喂食 10000、20000 和 40000 个孢子/只。蜜蜂的感染率和存活率存在显著的剂量依赖性差异。感染后 6、10 和 14 天，感染细胞与非感染细胞的比例增加。此外，对照组蜜蜂的咽腺蛋白浓度显著高于感染组。感染 40000 个孢子/只的蜜蜂的蛋白浓度最低。因此，从东方蜜蜂中分离出的被囊菌属 N. ceranae 能够感染另一种蜜蜂物种，损害咽腺蛋白的产生，并降低中华蜜蜂的存活率。
