Instituto de Fisiología, Biología Molecular y Neurociencias, Departamento de Biodiversidad y Biología Experimental, Universidad de Buenos Aires, Argentina.
Brain Res Bull. 2011 Jan 15;84(1):31-8. doi: 10.1016/j.brainresbull.2010.10.003. Epub 2010 Oct 20.
Perinatal asphyxia (PA) may cause long-term neurological and psychiatric diseases. We evaluated, by ethanolic phosphotungstic acid (E-PTA) staining, whether PA affects postsynaptic densities (PSDs), ultrastructure of neostriatum and hippocampus of 45-day-old post-PA male and female rats. PA was induced by placing the uterine horns containing the fetuses in a 37°C bath for 10, 15, 19 and 20 min and a 15°C bath for 20 min (hypothermia). Striatal synaptic disorganization and PSDs thickness increase were evident after 10 and 19 min of PA in male and female rats, respectively, but striatal female PSDs thickness was lower than in males. These changes were associated with increments of the PSDs area in both sexes at 19 and 20 min PA. Thickness and PSDs area from hippocampal PA males was affected more negatively than in females. Intrahypoxic hypothermia was able to protect the brain from effects of PA. In conclusion, early PA affects neostriatal and hippocampal PSDs in a time and sex-dependent manner, while hypothermia during asphyxia is able to prevent synaptic changes by providing protection from damage.
围产期窒息（PA）可能导致长期的神经和精神疾病。我们通过乙醇磷钨酸（E-PTA）染色评估了 PA 是否会影响 45 日龄雄性和雌性 PA 大鼠的纹状体和海马回的突触后密度（PSD）和超微结构。通过将包含胎儿的子宫角置于 37°C 的浴中 10、15、19 和 20 分钟以及 15°C 的浴中 20 分钟（体温过低）来诱导 PA。在雄性和雌性大鼠中，PA 10 分钟和 19 分钟后，纹状体的突触紊乱和 PSDs 厚度增加是明显的，但纹状体雌性 PSDs 厚度低于雄性。这些变化与 PA 19 和 20 分钟时 PSDs 面积的增加有关。PA 雄性的 PSDs 厚度和面积的影响比雌性更为负面。缺氧性低体温能够保护大脑免受 PA 的影响。总之，早期 PA 以时间和性别依赖的方式影响纹状体和海马回的 PSDs，而在窒息期间的低温能够通过提供损伤保护来防止突触变化。
