School of Health Sciences, Faculty of Medicine, Gunma University, Maebashi, Gunma, Japan.
Pathol Res Pract. 2010 Dec 15;206(12):816-20. doi: 10.1016/j.prp.2010.09.005. Epub 2010 Oct 20.
Human papillomavirus (HPV) L1 capsid protein (L1) is associated with the productive phase of HPV infection. However, the expression of L1 and its relationship to p16 expression, a surrogate marker for HPV infection, are unknown. We examined the relationship between L1 and p16 expression in cervical intraepithelial neoplasia. Tissues were divided into four categories: regressive lesions (n=48), progressive lesions (n=40), and randomly selected CIN1-2 (n=67) and CIN3 (n=44). P16 positivity in the progression cases was significantly higher than that in the regression cases, and p16 positivity in the CIN3 cases was significantly higher than that in any other categories. L1 positivity was not significant between each category. The staining pattern was divided into the following four groups: L1-/p16-, L1+/p16-, L1+/p16+, L1-/p16+. The L1-/p16- pattern was significantly associated with the regression nature in CIN1-2. Some CIN3 cases showing a feature of L1+/p16+, which are still HPV-productive, are likely to exist. The combination of both L1 and p16 may be useful in the evaluation of the progression risk of low-grade cervical dysplasia.
人乳头瘤病毒（HPV）L1 衣壳蛋白（L1）与 HPV 感染的增殖期相关。然而，L1 的表达及其与 p16 表达的关系，p16 是 HPV 感染的替代标志物，尚不清楚。我们研究了 L1 与宫颈上皮内瘤变（CIN）中 p16 表达的关系。组织分为四类：退行性病变（n=48）、进行性病变（n=40），以及随机选择的 CIN1-2（n=67）和 CIN3（n=44）。进展病例的 p16 阳性率明显高于退行性病变，CIN3 病例的 p16 阳性率明显高于其他任何类别。每个类别之间 L1 阳性率没有显著差异。染色模式分为以下四种：L1-/p16-、L1+/p16-、L1+/p16+、L1-/p16+。L1-/p16-模式与 CIN1-2 的退行性特征显著相关。一些表现为 L1+/p16+特征的 CIN3 病例仍具有 HPV 增殖性，可能存在。同时检测 L1 和 p16 可能有助于评估低级别宫颈上皮内瘤变的进展风险。
