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Real-time polymerase chain reaction for rapid detection of genes encoding SHV extended-spectrum β-lactamases.

作者信息

Alfaresi M S, Elkoush A A

机构信息

Department of Pathology & Laboratory Medicine, Zayed Military Hospital, PO Box 3740, Abu Dhabi, United Arab Emirates.

出版信息

Indian J Med Microbiol. 2010 Oct-Dec;28(4):332-6. doi: 10.4103/0255-0857.71827.

DOI:10.4103/0255-0857.71827
PMID:20966564
Abstract

PURPOSE

This study aimed to develop an improved method for the detection of bacterial SHV-type extended-spectrum β-lactamases (ESBLs).

MATERIALS AND METHODS

Our method was based on real-time polymerase chain reaction (PCR) in which the amplification of the product was monitored with a fluorescent probe. This method enabled the detection of bla SHV genes with high degrees of sensitivity and specificity.

RESULTS

Based on ESBL phenotyping methods and bla gene DNA sequencing, we identified 240 bla genes from 662 Enterobacteriaceae isolated from clinical culture specimens. Of these 240 isolates, 26 had the bla SHV-28 genotype and three had the bla SHV-1 genotype. With our new real-time PCR assay, we detected 29 out of 29 bla SHV genes in ESBL-producing isolates.

CONCLUSION

This method represents a powerful tool for epidemiological studies of SHV ESBLs. Furthermore, it has potential for use in diagnostic microbiology.

摘要

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