Gupta M, Singh N P, Kaur I R
Department of Microbiology, UCMS & GTB Hospital, Delhi, India.
Indian J Med Microbiol. 2010 Oct-Dec;28(4):363-5. doi: 10.4103/0255-0857.71816.
The aim of this study was to evaluate a nitrate reductase assay (NRA) for the direct detection of multidrug resistance (MDR) in Mycobacterium tuberculosis from 100 smear-positive sputum samples. The NRA results were compared with the reference proportion method for 100 sputum specimens for which comparable results were available. NRA results were obtained at day 7 for 61 specimens, results for 26 specimens were obtained at day 10, and the results for 13 specimens were obtained at day 14. Thus, 87% of NRA results were obtained in 10 days. NRA is a rapid, accurate, and cost-effective method for the detection of MDR in M. tuberculosis isolates as compared to the proportion method, which is time consuming. Therefore, NRA constitutes a useful tool for detection of tuberculosis drug resistance in low-resource countries with limited laboratory facilities due to its low-cost, ease of performance and lack of requirement of sophisticated equipment.
本研究的目的是评估一种硝酸盐还原酶测定法（NRA），用于直接检测100份涂片阳性痰标本中结核分枝杆菌的多重耐药性（MDR）。将NRA结果与100份痰标本的参考比例法进行比较，这些标本可获得可比结果。61份标本在第7天获得NRA结果，26份标本在第10天获得结果，13份标本在第14天获得结果。因此，87%的NRA结果在10天内获得。与耗时的比例法相比，NRA是一种快速、准确且经济高效的检测结核分枝杆菌分离株中MDR的方法。因此，由于NRA成本低、操作简便且无需复杂设备，它是在实验室设施有限的资源匮乏国家检测结核病耐药性的有用工具。
