神经元中轴突运输的实时可视化。

Real-time visualization of axonal transport in neurons.

作者信息

Osakada Yasuko, Cui Bianxiao

机构信息

Department of Chemistry, Stanford University, Stanford, CA, USA.

出版信息

Methods Mol Biol. 2011;670:231-43. doi: 10.1007/978-1-60761-744-0_16.

DOI:10.1007/978-1-60761-744-0_16

PMID:20967594

Abstract

The normal function of neurons depends on the integrity of microtubule-dependent transport of cellular materials and organelles to/from their cell bodies or axon terminus. In this chapter, we describe the design and implementation of a fluorescence imaging method to visualize axonal transport in neurons directly. We combine a pseudo total internal reflection microscopy, quantum dot fluorescence labeling, microfluidic neuronal culture chamber, and single molecule detection methods to achieve a high spatial and temporal resolution in tracking nerve growth factor transport in dorsal root ganglia neurons.

摘要

神经元的正常功能取决于细胞物质和细胞器在微管依赖下向/从其细胞体或轴突末端运输的完整性。在本章中，我们描述了一种荧光成像方法的设计与实施，以直接可视化神经元中的轴突运输。我们结合了伪全内反射显微镜、量子点荧光标记、微流控神经元培养室和单分子检测方法，在追踪背根神经节神经元中神经生长因子的运输时实现了高空间和时间分辨率。