Takeuchi T, Rechnitz G A
Department of Chemistry, University of Hawaii at Manoa, Honolulu 96822.
Bioconjug Chem. 1990 Jul-Aug;1(4):227-30. doi: 10.1021/bc00004a001.
A novel enzymatic ligand binding assay for biotin and its benzodiazepine conjugate is based on their binding to horseradish peroxidase-avidin conjugate (A-P) followed by the uptake of biotin-unsaturated A-P onto polystyrene beads coated with biotin-BSA. The detection limit is 1.3 x 10(-16) mol per tube (300 microL) with a 3.3 x 10(-12) M A-P solution and varies with the conjugate concentration employed. The coefficient of variation for 10 repetitive assays of 10(-15) mol of biotin is 6.22%.
一种用于生物素及其苯二氮䓬共轭物的新型酶促配体结合测定法，其基于它们与辣根过氧化物酶-抗生物素蛋白共轭物（A-P）的结合，随后将生物素-不饱和A-P摄取到涂有生物素-BSA的聚苯乙烯珠上。使用3.3×10⁻¹² M的A-P溶液时，每管（300 μL）的检测限为1.3×10⁻¹⁶ mol，并且随所用共轭物浓度而变化。对10⁻¹⁵ mol生物素进行10次重复测定的变异系数为6.22%。
