Koch T, Suenson E, Henriksen U, Buchardt O
Research Center for Medical Biotechnology, H. C. Orsted Institute, University of Copenhagen, Denmark.
Bioconjug Chem. 1990 Jul-Aug;1(4):296-304. doi: 10.1021/bc00004a012.
The intensive use of cleavable cross-linking reagents to study macromolecular biological interactions has shown a demand for optimizing these reagents in such a way that the involved macromolecules remain intact. The present work focuses on the development of selenium linkers that are cleavable by mild oxidation. The efficiency of cross-linking and subsequent cross-linker cleavage with a new series of such homo- or heterobifunctional cross-linking reagents have been tested in a simple model system, consisting of albumin and cytochrome c. Resultant, or residual, covalent complex formation is examined by SDS-polyacrylamide gel electrophoresis. From this work it can be concluded that diallyl selenides are readily cleaved by mild oxidation, whereas dialkyl selenides and benzyl alkyl selenides can only be cleaved when the alkyl part of the selenide has an electron-withdrawing group next to the beta-carbon from selenium.
大量使用可裂解交联试剂来研究大分子生物相互作用表明，需要以一种使所涉及的大分子保持完整的方式来优化这些试剂。目前的工作重点是开发可通过温和氧化裂解的硒连接体。已在由白蛋白和细胞色素c组成的简单模型系统中测试了一系列新的此类同双功能或异双功能交联试剂的交联效率以及随后的交联剂裂解效率。通过SDS-聚丙烯酰胺凝胶电泳检查共价复合物的形成情况。从这项工作可以得出结论，二烯丙基硒化物很容易被温和氧化裂解，而二烷基硒化物和苄基烷基硒化物只有在硒化物的烷基部分在距硒的β-碳相邻位置有一个吸电子基团时才能被裂解。
