Duzhak T G, Mishin V M, Liakhovich V V, Tsyrlov I B
Biokhimiia. 1990 Dec;55(12):2129-37.
Antibodies to mouse liver cytochrome P3-450 (anti-P3-450) and antibodies to rat liver cytochrome P-450d (anti-P-450d-c) inhibit the 0-deethylation of 7-ethoxyresorufin (ER) in liver microsomes of benz(a)pyrene-induced (BP) mice but do not inhibit the 0-deethylase activity in liver microsomes of BP-induced rats. Anti-P3-450 and anti-P-450c inhibit BP-hydroxylation in BP-induced mouse liver microsomes by 20%, but they do not inhibit this reaction at all in BP-induced rat liver microsomes. In a reconstituted monooxygenase system isolated cytochrome P3-450 metabolized 7-ER and BP. In contrast, its homologue, cytochrome P-450d, did not metabolize these substrates. The fraction containing cytochrome P1-450 metabolized 7-ER at a low rate and BP at a rate of 3.6 nmol product/min/nmol cytochrome. Western blot analysis with anti-P-450c + d revealed two bands in SDS-PAGE gels containing BP-induced mouse liver microsomes. The interaction of mouse liver BP-microsomes with anti-P3-450 and anti-P-450d-c was accompanied by the appearance of a single band (cytochrome P3-450).
针对小鼠肝脏细胞色素P3 - 450的抗体（抗P3 - 450）和针对大鼠肝脏细胞色素P - 450d的抗体（抗P - 450d - c）可抑制苯并(a)芘诱导的（BP）小鼠肝脏微粒体中7 - 乙氧基试卤灵（ER）的O - 脱乙基化反应，但不抑制BP诱导的大鼠肝脏微粒体中的O - 脱乙基酶活性。抗P3 - 450和抗P - 450c可使BP诱导的小鼠肝脏微粒体中的BP羟化反应抑制20%，但在BP诱导的大鼠肝脏微粒体中对该反应完全无抑制作用。在重组单加氧酶系统中，分离出的细胞色素P3 - 450可代谢7 - ER和BP。相比之下，其同源物细胞色素P - 450d不能代谢这些底物。含有细胞色素P1 - 450的组分以低速率代谢7 - ER，以3.6 nmol产物/分钟/ nmol细胞色素的速率代谢BP。用抗P - 450c + d进行的蛋白质免疫印迹分析在含有BP诱导的小鼠肝脏微粒体的SDS - PAGE凝胶中显示出两条带。小鼠肝脏BP微粒体与抗P3 - 450和抗P - 450d - c的相互作用伴随着一条单带（细胞色素P3 - 450）的出现。
