Department of Pharmacy, University of Peshawar, Peshawar 25120, Pakistan.
Fitoterapia. 2011 Mar;82(2):276-81. doi: 10.1016/j.fitote.2010.10.011. Epub 2010 Oct 20.
A new taxoid Taxawallin I (1) along with two known taxoids (2-3) were isolated from methanolic bark extract of Taxus wallichiana Zucc. Structural characterization was confirmed by mass and NMR spectral techniques. Taxawallin I exhibited significant in-vitro anticancer activity against HepG2, A498, NCI-H226 and MDR 2780AD cancer lines. Tubulin binding assay was performed to assess its tubulin binding activity. Molecular docking analysis was performed to study the potential binding mode inside the taxol binding site of β-tubulin.
从云南红豆杉树皮的甲醇提取物中分离得到了一种新的紫杉烷类化合物 Taxawallin I（1）以及两种已知的紫杉烷类化合物（2-3）。通过质谱和 NMR 光谱技术确认了其结构特征。Taxawallin I 对 HepG2、A498、NCI-H226 和 MDR 2780AD 癌细胞系表现出显著的体外抗癌活性。进行了微管蛋白结合实验来评估其微管蛋白结合活性。进行了分子对接分析以研究其在β-微管蛋白的紫杉醇结合位点内的潜在结合模式。
