Department for Bacteriology and Parasitology, Croatian Veterinary Institute, Savska cesta 143, Zagreb, Croatia.
Vet Parasitol. 2011 Jan 10;175(1-2):40-6. doi: 10.1016/j.vetpar.2010.09.026. Epub 2010 Oct 20.
A total of 131 faecal samples from 57 mammalian species housed at the zoo of Zagreb, Croatia, were tested for the presence of Giardia spp. cysts using epifluorescence microscopy. The overall prevalence (29%) was high, yet all animals were asymptomatic at the time of sampling. Positive samples were characterized by PCR and sequence analysis of both conserved and variable loci, for the identification of Giardia species and G. duodenalis assemblages and genotypes. Assemblages A and C were identified in Artiodactyla, assemblage B in Primates, Rodentia and Hyracoidea, and assemblages A, B, C and D, as well as Giardia microti, in Carnivora. Genotyping at the ITS1-5.8S-ITS2 region, at the triose phosphate isomerase, glutamate dehydrogenase and beta-giardin genes revealed extensive polymorphisms, particularly among assemblage B isolates. A phylogenetic analysis of concatenated sequences showed that isolates from captive mammals housed at the zoo are genetically different from isolates of human and domestic animal origin. This is the first survey in a zoological garden to include a molecular characterization of the parasite, and provides novel sequence data of G. duodenalis from many previously uncharacterized hosts.
从克罗地亚萨格勒布动物园饲养的 57 种哺乳动物的 131 份粪便样本中，使用荧光显微镜检测了隐孢子虫属囊包的存在。总体流行率（29%）较高，但所有动物在采样时均无症状。阳性样本通过 PCR 进行了特征描述，并对保守和可变基因座进行了序列分析，以鉴定隐孢子虫种和十二指肠贾第虫组合和基因型。在偶蹄目动物中鉴定出了组合 A 和 C，在灵长目动物、啮齿目动物和蹄兔目动物中鉴定出了组合 B，在食肉目动物中鉴定出了组合 A、B、C 和 D，以及微小隐孢子虫。在 ITS1-5.8S-ITS2 区域、磷酸丙糖异构酶、谷氨酸脱氢酶和β-微管蛋白基因的基因分型显示出广泛的多态性，特别是在组合 B 分离株中。串联序列的系统发育分析表明，动物园圈养哺乳动物的分离株与来自人类和家畜的分离株在遗传上不同。这是动物园首次进行寄生虫的分子特征描述，提供了许多以前未被描述的宿主的十二指肠贾第虫的新序列数据。
