雌激素、雷洛昔芬和左美诺昔芬对尿道平滑肌细胞 Rho 激酶信号分子表达的影响。
Effects of estrogen, raloxifene, and levormeloxifene on the expression of Rho-kinase signaling molecules in urethral smooth muscle cells.
机构信息
Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, California 94143, USA.
出版信息
Urology. 2010 Dec;76(6):1517.e6-11. doi: 10.1016/j.urology.2010.07.470. Epub 2010 Oct 23.
OBJECTIVES
To investigate the effects of estrogen, raloxifene, and levormeloxifene on the expression of Rho-kinase signaling molecules in urethral smooth muscle cells (USMCs).
METHODS
USMCs were isolated from female rats. Expression of calponin and estrogen receptors α (ERα) was detected by immunofluorescence staining. Cells were treated with estrogen, raloxifene, or levormeloxifene at 0, 1, 10, and 100 nmol/L for 48 h and then processed for Western blotting with antibodies against RhoA, Rho kinase I and II (Rock-I and Rock-II), myosin light chain (MLC), phosphorylated MLC, and β-actin. Protein expression was quantitated by densitometry, followed by statistical analysis with β-actin as control.
RESULTS
USMCs expressed calponin and ERα. Treatment of USMCs with estrogen, raloxifene or levormeloxifene resulted in decreased expression of RhoA, Rock-I, Rock-II, and p-MLC in a dosage-dependent manner.
CONCLUSIONS
Estrogen, raloxifene, and levormeloxifene may affect urinary continence by inhibiting the expression of Rho-kinase signaling molecules.
目的
研究雌激素、雷洛昔芬和左甲孕诺酮对尿道平滑肌细胞（USMC）中 Rho 激酶信号分子表达的影响。
方法
从雌性大鼠中分离 USMC。通过免疫荧光染色检测钙调蛋白和雌激素受体α（ERα）的表达。用 0、1、10 和 100 nmol/L 的雌激素、雷洛昔芬或左甲孕诺酮处理细胞 48 h，然后用抗 RhoA、Rho 激酶 I 和 II（Rock-I 和 Rock-II）、肌球蛋白轻链（MLC）、磷酸化 MLC 和 β-肌动蛋白的抗体进行 Western 印迹。通过密度计定量蛋白质表达，然后用β-肌动蛋白作为对照进行统计学分析。
结果
USMC 表达钙调蛋白和 ERα。雌激素、雷洛昔芬或左甲孕诺酮处理 USMC 可使 RhoA、Rock-I、Rock-II 和 p-MLC 的表达呈剂量依赖性降低。
结论
雌激素、雷洛昔芬和左甲孕诺酮可能通过抑制 Rho 激酶信号分子的表达来影响尿控。
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