Institute of Pesticide Science, Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, Northwest A&F University, Box 61, Xinong Road 22, Yangling, Shaanxi 712100, China.
Ecotoxicol Environ Saf. 2011 Mar;74(3):439-44. doi: 10.1016/j.ecoenv.2010.10.004.
The ultrastructural effects of ingested Celangulin V (CA-V), an insecticidal component extracted from the root bark of Chinese bittersweet (Celastrus angulatus Maxim), on the midgut epithelial cells of the oriental armyworm larva (Mythimna separata Walker) were studied. Transmission electron microscopy showed that CA-V could induce a severe, time-dependent cytotoxicity of the midgut epithelial cells, which had large cytoplasmic spaces, disrupted microvilli and swollen mitochondria. The cisternae of the rough endoplasmic reticulum were excessively dilated, vesiculated and then fragmented. The nuclei were pycnotic and contained one or two divided nucleoli and agglomerated chromatin. Numerous lysosome-like vacuoles and secretion granules were observed. Finally, the CA-V resulted in cell death by necrosis with plasma membrane lysis and the cytoplasm content leakage into the migdut lumen.
摄入从中国苦皮藤（Celastrus angulatus Maxim）根皮中提取的杀虫成分 Celangulin V（CA-V）对东方行军毛虫幼虫（Mythimna separata Walker）中肠上皮细胞的超微结构影响进行了研究。透射电子显微镜显示，CA-V 可诱导中肠上皮细胞产生严重的、时间依赖性的细胞毒性，导致细胞质空间增大、微绒毛破坏和线粒体肿胀。粗面内质网的池过度扩张、空泡化，然后碎裂。细胞核固缩，含有一个或两个分裂的核仁以及聚集的染色质。观察到大量溶酶体样空泡和分泌颗粒。最后，CA-V 通过坏死导致细胞膜裂解和细胞质内容物泄漏到中肠腔，从而导致细胞死亡。
