Szczech J
Department of Neurology, Laboratory of Neuropathology, Medical School Poznań, Poland.
Exp Pathol. 1990;40(3):169-78. doi: 10.1016/s0232-1513(11)80341-5.
Hypoxic hypoxia is used as one of the models for examining the unfavourable effect of oxygen deficit on the nervous system. Since some morphological studies concerning the effect of hypoxia on the central nervous system have given consideration to perivascular lesion zones and glia alterations in the white matter, the present evaluation of the effect of hypoxia has been directed to vascular endothelia of the white matter. Examinations were conducted on adult rats of the Wistar strain which were exposed to acute hypoxia by being placed in a gas mixture containing 2% O2 or to moderate hypoxia - in a gas mixture containing 7% O2. Experimental animals were sacrificed 4 and 24 h after hypoxia and 14 and 60 d after hypoxia. The behaviour of vascular endothelia after hypoxia was evaluated by karyometric and cytophotometric methods. The measurements show an edema of endothelial nuclei in capillaries of the cerebral white matter of the rat in the first 24 h after hypoxia accompanied by nuclear chromatin index alterations proportional to the intensity of hypoxia. The measurements of endothelial nuclei 14 and 60 d after hypoxia show a development of a degenerative process in these nuclei, manifested by their decreasing size and by nuclear chromatin concentration in their central parts. The morphometric and cytophotometric examinations point to long-term alterations induced by hypoxic hypoxia.
低氧性缺氧被用作研究氧缺乏对神经系统不利影响的模型之一。由于一些关于缺氧对中枢神经系统影响的形态学研究已经考虑到白质中的血管周围病变区域和神经胶质细胞改变，因此目前对缺氧影响的评估已针对白质的血管内皮。对成年Wistar品系大鼠进行了检查，将其置于含2%氧气的混合气体中使其暴露于急性缺氧状态，或将其置于含7%氧气的混合气体中使其暴露于中度缺氧状态。在缺氧后4小时和24小时以及缺氧后14天和60天处死实验动物。通过核测量法和细胞光度法评估缺氧后血管内皮的行为。测量结果显示，在缺氧后的头24小时内，大鼠脑白质毛细血管中的内皮细胞核出现水肿，同时核染色质指数改变与缺氧强度成比例。缺氧后14天和60天对内皮细胞核的测量显示，这些细胞核出现退行性变化，表现为细胞核大小减小以及核染色质在中央部分浓缩。形态测量和细胞光度检查表明低氧性缺氧会引起长期变化。
