Antosiewicz D
Institute of Botany, Faculty of Biology, University of Warsaw, Poland.
Folia Histochem Cytobiol. 1990;28(1-2):79-95.
The influence of a polish anticancer drug on the cell cycle using Allium test was studied. Methods of aceto-orcein squash slides, curve of labelled mitoses after 3H-thymidine incubation and cytophotometrics after Feulgen's reaction were employed. Ledakrin acts strongly antimitotically, but it does not block the cell cycle completely. The cytostatic activity of ledakrin results from its action on the interphase. The phases G1 and S are prolonged while M is unchanged after 6h incubation with ledakrin. During postincubation in water without ledakrin it was noted, at the beginning, that the mitotic activity decreases and it is brought about the lengthening of S and G2 phases. The duration of the cell cycle phases returns to the control level during further postincubation. The results of analysis of chromatin aberrations and the micronucleus test point to a mutagenic effect of ledakrin.
研究了一种波兰抗癌药物对细胞周期的影响，采用了大蒜试验。使用了醋洋红压片法、3H-胸腺嘧啶核苷孵育后的标记有丝分裂曲线以及福尔根反应后的细胞光度测定法。来达克林具有强烈的抗有丝分裂作用，但它并未完全阻断细胞周期。来达克林的细胞抑制活性源于其对间期的作用。与来达克林孵育6小时后，G1期和S期延长，而M期不变。在不含来达克林的水中孵育后，一开始观察到有丝分裂活性降低，这是由S期和G2期延长导致的。在进一步孵育期间，细胞周期各阶段的持续时间恢复到对照水平。染色质畸变分析和微核试验结果表明来达克林具有诱变作用。
