Department of Safety, Health and Environmental Engineering, Central Taiwan University of Science and Technology, Bei-tun District, Taichung, Taiwan.
Biodegradation. 2011 Jun;22(3):551-63. doi: 10.1007/s10532-010-9427-z. Epub 2010 Oct 23.
This study attempted to determine the optimal temperature and initial cultivation pH by conducting a series of batch tests in stirred-tank bioreactor using fructose-producing wastewater as an organic substrate. The bioreactor temperature was controlled at 35-55°C with an initial pH of 4-8. Hydrogen production efficiency was assessed using specific hydrogen production potential (SHPP) and the maximum specific hydrogen production rate (SHPR(m)). Experimental results indicated that temperature and initial pH markedly affected SHPP and SHPR(m), volatile fatty acids distribution as well as the ratio of butyrate/acetate (BHu/HAc). Two-fold higher SHPP and SHPR(m) were obtained at thermophilic condition (55°C) than those at mesophilic condition (35°C). The optimal initial pH was 6 for hydrogen production with peak values of SHPP of 166.8 ml-H(2)/g-COD and SHPR(m) of 26.7 ml-H(2)/g-VSS-h for fructose-processing wastewater. Molasses-processing wastewater had a higher SHPP (187.0 ml-H(2)/g-COD) and SHPR(m) (42.7 ml-H(2)/gVSS-h) than fructose-processing wastewater at pH 6. The DGGE profiles indicated that molasses-processing wastewater is a better substrate than fructose-processing wastewater for growth of hydrogen-producing bacteria due to the high staining intensity of bands.
本研究试图通过在搅拌槽生物反应器中使用产氢废水作为有机底物进行一系列分批试验来确定最佳温度和初始培养 pH 值。生物反应器温度控制在 35-55°C，初始 pH 值为 4-8。使用特定产氢潜力 (SHPP) 和最大比产氢速率 (SHPR(m)) 评估产氢效率。实验结果表明，温度和初始 pH 值显著影响 SHPP 和 SHPR(m)、挥发性脂肪酸分布以及丁酸/乙酸比 (BHu/HAc)。在嗜热条件（55°C）下获得的 SHPP 和 SHPR(m) 比中温条件（35°C）下高两倍。产氢的最佳初始 pH 值为 6，在该 pH 值下，产氢废水的 SHPP 峰值为 166.8 ml-H(2)/g-COD，SHPR(m) 峰值为 26.7 ml-H(2)/g-VSS-h。在 pH 值为 6 时，糖蜜处理废水的 SHPP（187.0 ml-H(2)/g-COD）和 SHPR(m)（42.7 ml-H(2)/g-VSS-h）均高于果糖处理废水。DGGE 图谱表明，由于条带的高染色强度，糖蜜处理废水是比果糖处理废水更好的产氢细菌生长底物。
