Zuo J, Zuo Y, Zhang W, Chen J
Department of Environmental Science and Engineering, Tsinghua University, Beijing, 100084, PR China.
Water Sci Technol. 2005;52(10-11):31-9.
Anaerobic bio-hydrogen production is the focus in the field of bio-energy resources. In this paper, a series of batch experiments were conducted to investigate the effects of several factors on anaerobic bio-hydrogen producing process carried out by pre-heated river sediments. The results showed that several factors such as substrate and its concentration, temperature and the initial pH value could affect the anaerobic bio-hydrogen production in different levels. At 35 degrees C and the initial pH of 6.5, using glucose of 20,000 mg COD/L as substrate, the highest hydrogen production of 323.8 ml-H2/g TVS in a 100 ml batch reactor was reached, the specific hydrogen production rate was 37.7 ml-H2/g TVS h, and the hydrogen content was 51.2%. Thereafter using the same pre-heated river sediments as seed sludge, continuous anaerobic bio-hydrogen production was achieved successfully in a lab-scale CSTR with gas-separator. At the organic loading rate of 36 kg COD/m3 d, the highest hydrogen production was 6.3-6.7 l-H2/l-reactor d, the specific hydrogen production was 1.3-1.4 mol-H2/mol-glucose, and the hydrogen content in the gas was 52.3%. The effluent of the bio-reactor contained some small molecular organics, mainly including ethanol, acetate, butyrate and their molar proportion is 1:1:0.6.
厌氧生物制氢是生物能源领域的研究热点。本文通过一系列批次实验，研究了几个因素对预热河流沉积物厌氧生物制氢过程的影响。结果表明，底物及其浓度、温度和初始pH值等因素会在不同程度上影响厌氧生物制氢。在35℃、初始pH值为6.5的条件下，以20000mg COD/L的葡萄糖为底物，在100ml批次反应器中达到了最高产氢量323.8ml-H₂/g TVS，比产氢速率为37.7ml-H₂/g TVS h，氢气含量为51.2%。此后，以相同的预热河流沉积物作为接种污泥，在实验室规模的带有气液分离器的连续搅拌釜式反应器（CSTR）中成功实现了连续厌氧生物制氢。在有机负荷率为36kg COD/m³ d时，最高产氢量为6.3 - 6.7l-H₂/l-反应器 d，比产氢量为1.3 - 1.4mol-H₂/mol-葡萄糖，气体中的氢气含量为52.3%。生物反应器的出水含有一些小分子有机物，主要包括乙醇、乙酸、丁酸，其摩尔比例为1:1:0.6。
