Gencoglan Gulsum, Tosun Murat
Department of Dermatology, Medical Faculty of Celal Bayar University, Manisa, Turkey.
Cutan Ocul Toxicol. 2011 Mar;30(1):55-60. doi: 10.3109/15569527.2010.521537. Epub 2010 Oct 26.
The results of studies on the effects of retinoids on spermatogenesis are controversial.
We evaluated time- and dose-dependent effects of isotretinoin on spermatogenic activity by apoptosis, cyclin D1, E2F, p53 expressions, and Johnsen's scores.
The rats were divided into three groups. In the 1st group (n = 18), 1 mg/mL/day and in the 2nd group (n = 18) 2 mg/mL/day isotretinoin were administered for 21 days. Flower oil was given to the 3rd (n = 6) control group. On the 7th (groups 1a and 2a), 14th (groups 1b and 2b), and 21st (groups 1c and 2c) days, six rats from the 1st group and six rats from the 2nd group were sacrificed and bilateral orchiectomy was done.
The number of cyclin D1 and E2F-positive cells decreased nonsignificantly parallel to days in the 1st group, whereas there was a statistically significant decrease in the 2nd group for the same cells. The p53-positive cells in groups 1c and 2c were increased significantly.
Further studies on the effects of retinoids on spermatogenesis should be conducted. It may be wise to administer contraception to male patients, especially during high-dose and long-term retinoid therapy.
关于维甲酸对精子发生作用的研究结果存在争议。
我们通过凋亡、细胞周期蛋白D1、E2F、p53表达以及约翰森评分来评估异维甲酸对生精活性的时间和剂量依赖性影响。
将大鼠分为三组。第一组（n = 18）给予1毫克/毫升/天的异维甲酸，第二组（n = 18）给予2毫克/毫升/天的异维甲酸，持续21天。第三组（n = 6）为对照组，给予花油。在第7天（1a组和2a组）、第14天（1b组和2b组）和第21天（1c组和2c组），处死第一组的6只大鼠和第二组的6只大鼠，并进行双侧睾丸切除术。
第一组中细胞周期蛋白D1和E2F阳性细胞数量随时间非显著平行减少，而第二组中相同细胞数量有统计学意义的减少。1c组和2c组中p53阳性细胞显著增加。
应进一步开展关于维甲酸对精子发生作用的研究。对男性患者，尤其是在高剂量和长期维甲酸治疗期间进行避孕可能是明智的。
