Veselá I, Kotasová H, Jankovská S, Procházková J, Pacherník J
Institute of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic.
Folia Biol (Praha). 2010;56(4):165-72.
The leukaemia inhibitory factor is a cytokine that exhibits pleiotropic activities in a wide range of cell types. There are evidences that leukaemia inhibitory factor-regulated signalling pathways are involved in cardiomyogesis and maintenance of cardiomyocytes. In the present work we studied the effect of leukaemia inhibitory factor on cardiomyogenesis of embryonic stem cells together with the role of serum-born factors. We showed that leukaemia inhibitory factor had an inhibitory effect during both the induction and progression phases of cardiomyogenesis of embryonic stem cells. The leukaemia inhibitory factor-mediated inhibition of cardiomyogenesis was abolished by inhibitors of STAT3 activity. These results suggest that leukaemia inhibitory factor- activated STAT3 is responsible for the inhibition of cardiomyogenesis in embryonic stem cells.
白血病抑制因子是一种细胞因子，在多种细胞类型中表现出多效性活性。有证据表明，白血病抑制因子调节的信号通路参与心肌细胞生成和心肌细胞的维持。在本研究中，我们研究了白血病抑制因子对胚胎干细胞心肌细胞生成的影响以及血清源性因子的作用。我们发现，白血病抑制因子在胚胎干细胞心肌细胞生成的诱导期和进展期均具有抑制作用。STAT3活性抑制剂消除了白血病抑制因子介导的心肌细胞生成抑制作用。这些结果表明，白血病抑制因子激活的STAT3负责抑制胚胎干细胞中的心肌细胞生成。
