Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, PR China.
Fish Shellfish Immunol. 2011 Jan;30(1):234-40. doi: 10.1016/j.fsi.2010.10.013. Epub 2010 Oct 23.
The cDNA encoding an extracellular copper zinc superoxide dismutase (LvECSOD) was cloned from the hepatopancreas of white shrimp Litopenaeus vannamei. It consisted of 915 bp nucleotides with an open reading frame corresponding to a deduced protein of 178 amino acids. The LvECSOD contains a putative signal peptide of 16 amino acids, two potential N-linked glycosylation sites (N(115)GTA and N(135)ITG) and a copper zinc superoxide dismutase family signature sequence (G(162)NAGaRvACctI(173)). It was found that four copper binding sites, four zinc binding sites and two cysteines involving in the formation of the disulfide bridge were conserved in the protein. LvECSOD shared 33-58% identity to ECSODs from other organisms. Expression analysis revealed that LvECSOD mRNA was widely distributed in all the tissues examined. When the shrimp challenged with Vibrio alginolyticus or white spot syndrome virus (WSSV), expression of LvECSOD mRNA in the hepatopancreas and hemocytes was mediated responsively. Our results suggested that LvECSOD was implicated in the immune response induced by V. alginolyticus and WSSV.
从凡纳滨对虾（Litopenaeus vannamei）的肝胰腺中克隆出编码细胞外铜锌超氧化物歧化酶（LvECSOD）的 cDNA。它由 915 个核苷酸组成，开放阅读框对应于 178 个氨基酸的推断蛋白。LvECSOD 包含一个假定的 16 个氨基酸的信号肽，两个潜在的 N-连接糖基化位点（N(115)GTA 和 N(135)ITG）和一个铜锌超氧化物歧化酶家族特征序列（G(162)NAGaRvACctI(173)）。研究发现，该蛋白中存在四个铜结合位点、四个锌结合位点和两个涉及形成二硫键的半胱氨酸。LvECSOD 与其他生物体的 ECSOD 具有 33-58%的同源性。表达分析显示，LvECSOD mRNA 在所有检测的组织中广泛表达。当虾受到副溶血弧菌或白斑综合征病毒（WSSV）的挑战时，肝胰腺和血细胞中 LvECSOD mRNA 的表达受到响应性调节。我们的结果表明，LvECSOD 参与了副溶血弧菌和 WSSV 诱导的免疫反应。
