The Institute for Advanced Materials & Nano Biomedicine, Tongji University, Shanghai 200092, China.
J Colloid Interface Sci. 2011 Jan 15;353(2):426-32. doi: 10.1016/j.jcis.2010.09.084. Epub 2010 Oct 25.
Bifunctional nanoparticles with highly fluorescence and decent magnetic properties have been widely used in biomedical application. In this study, highly fluorescent magnetic nanoparticles (FMNPs) with uniform size of ca. 40 nm are prepared by encapsulation of both magnetic nanoparticles (MNPs) and shell/core quantum dots (QDs) with well-designed shell structure/compositions into silica matrix via a one-pot reverse microemulsion approach. The spectral analysis shows that the FMNPs hold high fluorescent quantum yield (QY). The QYs and saturation magnetization of the FMNPs can be regulated by varying the ratio of the encapsulated QDs to MNPs. Moreover, the surface of the FMNPs can be modified to offer chemical groups for antibody conjugation for following use in target-enrichment and subsequent fluorescent detection. The in vitro immunofluorescence assay and flow cytometric analysis indicate that the bifunctional FMNPs-antibody bioconjugates are capable of target-enrichment, magnetic separation and can also be used as alternative fluorescent probes on flow cytometry for biodetection.
具有高荧光和良好磁性的双功能纳米粒子已被广泛应用于生物医学领域。在这项研究中，通过一锅反相微乳液法，将具有均匀尺寸约为 40nm 的磁性纳米粒子（MNPs）和壳/核量子点（QDs）的壳层结构/组成封装到二氧化硅基质中，制备出具有高荧光量子产率（QY）的高荧光磁性纳米粒子（FMNPs）。光谱分析表明，FMNPs 具有高荧光量子产率（QY）。通过改变封装的 QDs 与 MNPs 的比例，可以调节 FMNPs 的 QY 和饱和磁化强度。此外，FMNPs 的表面可以进行修饰，提供用于抗体偶联的化学基团，用于后续的靶向富集和随后的荧光检测。体外免疫荧光分析和流式细胞术分析表明，双功能 FMNPs-抗体偶联物能够进行靶向富集、磁性分离，也可以用作流式细胞术生物检测的替代荧光探针。
