Quantitative Biology Center, Riken, Furuedai 6-2-3, Suita, Osaka 565-0874, Japan.
Biomaterials. 2012 Nov;33(33):8486-94. doi: 10.1016/j.biomaterials.2012.07.051. Epub 2012 Aug 17.
Multilayered, core/shell nanoprobes (MQQ-probe) based on magnetic nanoparticles (MNPs) and quantum dots (QDs) have been successfully developed for multimodality tumor imaging. This MQQ-probe contains Fe(3)O(4) MNPs, visible-fluorescent QDs (600 nm emission) and near infrared-fluorescent QDs (780 nm emission) in multiple silica layers. The fabrication of the MQQ-probe involves the synthesis of a primer Fe(3)O(4) MNPs/SiO(2) core by a reverse microemulsion method. The MQQ-probe can be used both as a fluorescent probe and a contrast reagent of magnetic resonance imaging. For breast cancer tumor imaging, anti-HER2 (human epidermal growth factor receptor 2) antibody was conjugated to the surface of the MQQ-probe. The specific binding of the antibody conjugated MQQ-probe to the surface of human breast cancer cells (KPL-4) was confirmed by fluorescence microscopy and fluorescence-activated cell sorting analysis in vitro. Due to the high tissue permeability of near-infrared (NIR) light, NIR fluorescence imaging of the tumor mice (KPL-4 cells transplanted) was conducted by using the anti-HER2 antibody conjugated MQQ-probe. In vivo multimodality images of breast tumors were successfully taken by NIR fluorescence and T(2)-weighted magnetic resonance. Antibody conjugated MQQ-probes have great potential to use for multimodality imaging of cancer tumors in vitro and in vivo.
基于磁性纳米粒子（MNPs）和量子点（QDs）的多层核/壳纳米探针（MQQ-探针）已成功开发用于多模态肿瘤成像。这种 MQQ-探针包含 Fe(3)O(4) MNPs、可见荧光 QDs（发射 600nm）和近红外荧光 QDs（发射 780nm），分布在多层二氧化硅中。MQQ-探针的制备涉及通过反相微乳液法合成 primer Fe(3)O(4) MNPs/SiO(2) 核。MQQ-探针既可以用作荧光探针，也可以用作磁共振成像的对比试剂。用于乳腺癌肿瘤成像时，将抗 HER2（人表皮生长因子受体 2）抗体偶联到 MQQ-探针的表面。通过荧光显微镜和体外荧光激活细胞分选分析证实了抗体偶联的 MQQ-探针与人类乳腺癌细胞（KPL-4）表面的特异性结合。由于近红外（NIR）光的组织穿透率高，因此使用抗 HER2 抗体偶联的 MQQ-探针对肿瘤小鼠（移植 KPL-4 细胞）进行了 NIR 荧光成像。通过 NIR 荧光和 T(2)-加权磁共振成功获得了乳腺癌肿瘤的体内多模态图像。抗体偶联的 MQQ-探针具有用于癌症肿瘤的体外和体内多模态成像的巨大潜力。
