State Key Laboratory of Food Science and Technology, Nanchang University, 235, Nanjing East Road, Nanchang 330047, Jiangxi, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2011 Jan;78(1):238-42. doi: 10.1016/j.saa.2010.09.028. Epub 2010 Oct 8.
A spectrophotometric method for the simultaneous determination of two herbicides, atrazine and cyanazine, is described for the first time based on their reaction with p-aminoacetophenone in the presence of pyridine in hydrochloric acid medium. The absorption spectra were measured in the wavelength range of 400-600 nm. The optimized method indicated that individual analytes followed Beer's law in the concentration ranges for atrazine and cyanazine were 0.2-3.5 mg L(-1) and 0.3-5.0 mg L(-1), and the limits of detection for atrazine and cyanazine were 0.099 and 0.15 mg L(-1), respectively. The original and first-derivative absorption spectra of the binary mixtures were performed as a pre-treatment on the calibration matrices prior to the application of chemometric models such as classical least squares (CLS), principal component regression (PCR), partial least squares (PLS). The analytical results obtained by using these chemometric methods were evaluated on the basis of percent relative prediction error and recovery. It was found that the application of PCR and PLS models for first-derivative absorbance data gave the satisfactory results. The proposed methods were successfully applied for the simultaneous determination of the two herbicides in several food samples.
首次基于在盐酸介质中吡啶存在下对氨基苯乙酮与两种除草剂莠去津和氰草津的反应，描述了一种同时测定这两种除草剂的分光光度法。在 400-600nm 的波长范围内测量吸收光谱。优化后的方法表明，在莠去津和氰草津的浓度范围内，各分析物均遵循比尔定律，莠去津和氰草津的检测限分别为 0.099 和 0.15mg/L。对二元混合物的原始和一阶导数吸收光谱进行了预处理，然后应用化学计量模型（如经典最小二乘法（CLS）、主成分回归（PCR）、偏最小二乘法（PLS））处理校准矩阵。基于相对预测误差和回收率评估了使用这些化学计量方法获得的分析结果。结果表明，应用 PCR 和 PLS 模型对一阶导数吸光度数据进行处理可获得满意的结果。该方法成功地应用于几种食品样品中两种除草剂的同时测定。
