Department of Surgery, University of Louisville, 550 South Jackson Street, Louisville, Kentucky, 40292, USA.
Inflamm Res. 2011 Apr;60(4):337-45. doi: 10.1007/s00011-010-0273-6. Epub 2010 Oct 26.
To investigate liver damage and abscess formation in murine, secondary peritonitis.
Male C57BL/6 mice.
Intraperitoneal injection with 10(3) CFU Klebsiella pneumoniae and treatment with gentamicin 5 mg/kg/day (BID), subcutaneously.
Animals were killed at 12, 24, 48 and 72 h after infection. Bacterial burden was determined in the blood and the liver. Liver abscess formation was assessed macroscopically and microscopically. Plasma levels of alkaline phosphatase (ALP) and alanine transaminase (ALT) were measured. Polymorphonuclear leukocyte (PMN) accumulation was assessed via tissue myeloperoxidase (MPO) concentrations. Liver interleukin-10 (IL-10) levels were determined by ELISA.
K. pneumoniae was detectable in the blood and the liver at 12 h after infection. Liver abscess formation was visible earliest at 24 h after infection and most pronounced within the right liver lobes. ALP and ALT levels peaked at 12 and 24 h after infection, respectively. MPO was elevated in the right and left liver lobes at 12 h but only in the right lobes at 48 h after infection, compared to tissue levels in naïve mice. Liver IL-10 concentrations were not significantly increased.
Peritonitis led to liver injury and abscess formation but did not significantly affect tissue concentrations of anti-inflammatory IL-10.
研究小鼠继发性腹膜炎中的肝损伤和脓肿形成。
雄性 C57BL/6 小鼠。
用 10(3)CFU 肺炎克雷伯菌腹腔内注射，并每天用 5mg/kg 庆大霉素（BID）皮下治疗。
感染后 12、24、48 和 72 小时处死动物。测定血液和肝脏中的细菌负荷。评估肝脓肿形成的宏观和微观情况。测量血浆碱性磷酸酶（ALP）和丙氨酸转氨酶（ALT）水平。通过组织髓过氧化物酶（MPO）浓度评估多形核白细胞（PMN）的积累。通过 ELISA 测定肝白介素-10（IL-10）水平。
感染后 12 小时可在血液和肝脏中检测到肺炎克雷伯菌。肝脓肿形成最早在感染后 24 小时可见，右肝叶最明显。ALP 和 ALT 水平分别在感染后 12 和 24 小时达到峰值。与未感染的小鼠相比，感染后 12 小时右、左肝叶的 MPO 升高，但仅在感染后 48 小时右肝叶升高。肝组织中 IL-10 浓度无明显升高。
腹膜炎导致肝损伤和脓肿形成，但对抗炎性 IL-10 组织浓度没有明显影响。
