Pediatric Allergy & Immunology, Faculty of Medicine, Marmara University, Istanbul, Turkey.
Pediatr Allergy Immunol. 2010 Nov;21(7):1059-63. doi: 10.1111/j.1399-3038.2010.01051.x.
To determine the optimal time of Bacillus Calmette-Guerin (BCG) vaccination for induction of Th1 immunity, we measured the interferon (IFN)-γ and interleukin (IL)-10 secretion in purified protein derivative (PPD)-stimulated peripheral blood mononuclear cell (PBMC) cultures in newborns vaccinated at birth or 2nd month of life. Moreover, role of CD4(+) CD25(+) T cells was studied by depletion assay at 8th month. Nineteen term and healthy newborns were randomized into two groups: Group I composed of 10 newborns vaccinated with BCG at birth and the remaining 9 (group II) at 2nd month of life. PBMCs were isolated at birth, 2nd and 8th months of age, and PPD-stimulated IL-10, 5 and IFN-γ secretion were assessed. The same measurements were repeated for IL-10 and IFN-γ after the depletion of CD4(+) CD25(+) T cells at the 8th month. Children vaccinated at birth demonstrated higher PPD-stimulated IFN-γ and IL-10 levels at 2 months of age when compared to non-vaccinated ones (p = 0.038 and p = 0.022, respectively), whereas at 8 months, no significant differences were detected between the two groups. Moreover, CD4(+) CD25(+)-depleted T-cell cultures resulted in lower PPD-stimulated IL-10 levels in those vaccinated at birth when compared to non-depleted condition at the 8th month (p < 0.001). BCG at birth upregulated PPD-stimulated IFN-γ secretion at the 2nd month and remained still detectable at 8 month after the vaccination, whereas those vaccinated at the 2nd month of life lacked that increase in IFN-γ response at the same time-point. Furthermore, depletion assays suggest that CD4(+) CD25(+) T cells are involved in PPD-stimulated IL-10 secretion in response to BCG vaccination.
为了确定卡介苗（BCG）接种诱导 Th1 免疫的最佳时间，我们测量了出生时或 2 个月时接种 BCG 的新生儿外周血单个核细胞（PBMC）培养物中纯化蛋白衍生物（PPD）刺激的干扰素（IFN）-γ和白细胞介素（IL）-10 的分泌。此外，在 8 个月时通过耗竭测定研究了 CD4+CD25+T 细胞的作用。19 名足月和健康的新生儿被随机分为两组：第 I 组由 10 名出生时接种 BCG 的新生儿组成，其余 9 名（第 II 组）在 2 个月时接种。在出生时、第 2 个月和第 8 个月分离 PBMC，并评估 PPD 刺激的 IL-10、5 和 IFN-γ的分泌。在第 8 个月时耗竭 CD4+CD25+T 细胞后，重复了相同的 IL-10 和 IFN-γ测量。与未接种疫苗的婴儿相比，出生时接种疫苗的婴儿在 2 个月时 PPD 刺激的 IFN-γ和 IL-10 水平更高（p = 0.038 和 p = 0.022），而在 8 个月时，两组之间未检测到显著差异。此外，与未耗竭条件相比，出生时接种疫苗的婴儿在第 8 个月时 CD4+CD25+-耗竭 T 细胞培养物中 PPD 刺激的 IL-10 水平较低（p <0.001）。出生时接种 BCG 可在第 2 个月上调 PPD 刺激的 IFN-γ分泌，并在接种后 8 个月仍可检测到，而在第 2 个月接种疫苗的婴儿在同一时间点缺乏 IFN-γ反应的增加。此外，耗竭测定表明，CD4+CD25+T 细胞参与了 BCG 接种后 PPD 刺激的 IL-10 分泌。
