Department of Cardiothoracic Surgery, Second Affiliated Hospital, Zhejiang University, Hangzhou, China.
Acta Biochim Biophys Sin (Shanghai). 2010 Nov;42(11):827-33. doi: 10.1093/abbs/gmq091.
Polyphyllin I (PPI), a small molecular monomer extracted from Rhizoma of Paris polyphyllin, shows strong anticancer effects in previous study. Human lung adenocarcinoma A549 cells, human lung squamous cell carcinoma SK-MES-1 cells, and human lung large cell carcinoma H460 cells were cultured and then treated with PPI. Cell proliferation and apoptosis were measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, flow cytometry, western blot analysis, and DNA ladder. Athymic nude mice bearing tumors were injected with PPI, and tumor growth was recorded. Our results showed that PPI significantly inhibited the proliferation of three non-small cell lung cancer (NSCLC) cell lines, with the inhibitory concentrations (IC50) of 1.24, 2.40, and 2.33 μg/ml for A549, H460, and SK-MES-1 cells, respectively. After being treated with 2.5 µg/ml of PPI for 24 h, the apoptotic rate of A549 cells was 39.68%, which was remarkably higher than that of the control. Tumor growth was significantly inhibited in the PPI-treated group compared with the group treated with cisplatin (DDP) or PBS in the nude mice. PPI exhibits antitumor ability in NSCLC cells in vitro and in vivo, which might be related to the apoptosis induced by PPI.
重楼苷 I（PPI）是从云南重楼中提取的小分子单体，在先前的研究中表现出很强的抗癌作用。培养人肺腺癌细胞 A549、人肺鳞癌细胞 SK-MES-1 和人肺大细胞癌细胞 H460，用 PPI 处理后，通过 3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐（MTT）比色法、流式细胞术、Western blot 分析和 DNA 梯带分析检测细胞增殖和凋亡。将荷瘤裸鼠注射 PPI，并记录肿瘤生长情况。结果表明，PPI 显著抑制三种非小细胞肺癌（NSCLC）细胞系的增殖，其对 A549、H460 和 SK-MES-1 细胞的抑制浓度（IC50）分别为 1.24、2.40 和 2.33μg/ml。用 2.5μg/ml 的 PPI 处理 24 h 后，A549 细胞的凋亡率为 39.68%，明显高于对照组。与顺铂（DDP）或 PBS 处理组相比，荷瘤裸鼠中 PPI 治疗组的肿瘤生长明显受到抑制。PPI 在体外和体内对 NSCLC 细胞具有抗肿瘤作用，这可能与 PPI 诱导的细胞凋亡有关。
