Silva Júnior J G, Garcia M A, Machado O L, Grassiano D M, Domont G B
Departamento de Bioquímica, Universidade Federal do Rio de Janeiro.
An Acad Bras Cienc. 1990 Mar;62(1):17-24.
A glycoprotein, RC-13, isolated from Ricinus communis seeds was reduced, S-alkylated and cleaved by trypsin. The tryptic digest was fractionated by ion-exchange chromatography and a glycopeptide was isolated and purified by high-voltage paper electrophoresis. When submitted to amino acid and carbohydrate analyses this major glycopeptide showed the following chemical composition: Lys1, Asp1, Thr2, Ser4, Glu1, Pro2, Gly2, Ala2, Val2, GlcN6, Man6 and Gal8. Hydrazynolysis positioned Ser as the C-terminal residue. It is postulated that this glycopeptide belongs to the C-terminal region of the allergen.
从蓖麻籽中分离出的一种糖蛋白RC - 13经还原、S - 烷基化处理后，用胰蛋白酶进行裂解。胰蛋白酶消化产物通过离子交换色谱法进行分离，一种糖肽通过高压纸电泳法进行分离和纯化。对这种主要糖肽进行氨基酸和碳水化合物分析时，其化学组成如下：赖氨酸1个、天冬氨酸1个、苏氨酸2个、丝氨酸4个、谷氨酸1个、脯氨酸2个、甘氨酸2个、丙氨酸2个、缬氨酸2个、氨基葡萄糖6个、甘露糖6个和半乳糖8个。肼解确定丝氨酸为C末端残基。据推测，这种糖肽属于过敏原的C末端区域。
