Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan.
Pharmacology. 2010;86(5-6):273-80. doi: 10.1159/000320769. Epub 2010 Oct 27.
This study examined the inhibitory effects of papaverine on twitches directly elicited by electrical stimulation of the mouse diaphragm. Papaverine (3-100 μM) inhibited twitches in a dose-dependent manner. Papaverine increased the cyclic adenosine monophosphate (cAMP) but not cyclic guanosine monophosphate (cGMP) content. IBMX, Db-cAMP and 8-br-cGMP did not affect twitches, whereas verapamil and NaCN inhibited twitches. Increases in extracellular Ca²+ removed the twitch inhibition caused by verapamil but not that caused by papaverine. Papaverine (30 and 100 μM) and NaCN (1 mM) decreased creatine phosphate and ATP contents. These results suggest that the relaxing effects of papaverine on mouse diaphragm are mainly due to inhibition of aerobic energy metabolism.
本研究考察了罂粟碱对电刺激小鼠膈神经直接引起的抽搐的抑制作用。罂粟碱（3-100 μM）呈剂量依赖性抑制抽搐。罂粟碱增加环磷酸腺苷（cAMP）但不增加环鸟苷酸（cGMP）的含量。IBMX、Db-cAMP 和 8-br-cGMP 不影响抽搐，而维拉帕米和 NaCN 抑制抽搐。增加细胞外 Ca²+ 去除了维拉帕米引起的抽搐抑制，但不影响罂粟碱引起的抽搐抑制。罂粟碱（30 和 100 μM）和 NaCN（1 mM）降低磷酸肌酸和 ATP 的含量。这些结果表明，罂粟碱对小鼠膈的松弛作用主要归因于抑制有氧能量代谢。
