Frazier J S, Oates E L, Salata K F, Jacobowitz D M
Laboratory of Clinical Science, National Institute of Mental Health, Bethesda, Maryland 20892.
Appl Theor Electrophor. 1990;1(4):197-200.
Serum samples from patients with type 1 (insulin-dependent) diabetes mellitus and controls were incubated with two-dimensional Western immunoblots of pancreas and other tissues. Two out of 26 (8%) of the diabetics and 0 out of 45 of the controls demonstrated reactivity against four pancreas-specific proteins with identical molecular weights of 29,000 daltons and different isoelectric points ranging from pH 7.0-8.0. It is concluded that 29 Kd autoantibody reactivity is not a major marker for type 1 diabetes, but may help identify a subgroup of type 1 diabetics.
将1型（胰岛素依赖型）糖尿病患者和对照组的血清样本与胰腺及其他组织的二维蛋白质免疫印迹进行孵育。26名糖尿病患者中有2名（8%），45名对照组患者中无一例对四种胰腺特异性蛋白质呈现反应性，这些蛋白质分子量均为29,000道尔顿，等电点不同，范围在pH 7.0 - 8.0之间。得出的结论是，29 Kd自身抗体反应性不是1型糖尿病的主要标志物，但可能有助于识别1型糖尿病患者的一个亚组。
