Meslemani Danny, Yaremchuk Kathleen, Rontal Michael
Department of Otolaryngology, Henry Ford Hospital, Detroit, MI 48202, USA.
Ear Nose Throat J. 2010 Oct;89(10):496-504.
We conducted a prospective observational study of tracheostomy tubes with three primary goals: (1) to identify the presence and location of bacterial biofilms on adult tubes, (2) to determine how soon after insertion of a tube the presence of a biofilm could be identified, and (3) to identify the bacterial organisms that formed the biofilms on these tubes. Our study materials consisted of 7 adult tracheostomy tubes that had been changed during a routine outpatient clinic visit or hospital consultation. The tubes were examined for the presence of biofilms on the posterior aspect of the outer cannula; also, specimens were obtained from the posterosuperior aspect of the cuff on the 3 tubes that had a cuff. Samples of 2 to 3 mm were taken from each site and analyzed by scanning electron microscopy. Bacterial biofilms were found on 4 of the 7 tubes; they were present on the outer cannula of 3 cuffless tubes that had been inserted 14 days, 4 months, and 2 years previously and on the cuff of 1 tube that had been inserted 10 days previously. The biofilms were composed of gram-positive cocci in pairs that were likely consistent with Staphylococcus epidermidis.
我们对气管造口管进行了一项前瞻性观察研究，主要有三个目标：（1）确定成人气管造口管上细菌生物膜的存在及位置；（2）确定插管后多久能够识别生物膜的存在；（3）识别在这些气管造口管上形成生物膜的细菌种类。我们的研究材料包括7根成人气管造口管，这些气管造口管是在常规门诊就诊或医院会诊期间更换的。检查这些气管造口管外插管后侧是否存在生物膜；此外，从3根带有袖带的气管造口管的袖带后上方获取标本。从每个部位采集2至3毫米的样本，并用扫描电子显微镜进行分析。在7根气管造口管中的4根上发现了细菌生物膜；它们存在于3根无袖带气管造口管的外插管上，这3根气管造口管分别于14天、4个月和2年前插入，以及1根于10天前插入的气管造口管的袖带上。生物膜由成对的革兰氏阳性球菌组成，可能与表皮葡萄球菌一致。
