Ragot M, Sari J C, Belaich J P
Biochim Biophys Acta. 1977 Oct 25;499(3):421-31. doi: 10.1016/0304-4165(77)90073-3.
Enthalpic variations in the formation of adenine nucleotide-manganese complexes, as measured by microcalorimetry, are reported. All the results are obtained in the temperature range 6--30 degrees C at I =0.2 and pH values 7.00 or 7.50. All the reactions are endothermic and the deltaH values increase with the length of the phosphate chain and with temperature. The deltaH values are compared with those previously obtained for adenine nucleotide-manganesium complexes. The comparison between calorimetric and potentiometric deltaH values is made. The divergence observed between these results at low temperature leads us to assume the formation of nucleotide aggregates induced by the presence of manganese ions. This hypothesis is confirmed by differential ultraviolet spectra.
本文报道了通过微量量热法测量的腺嘌呤核苷酸 - 锰配合物形成过程中的焓变。所有结果均在I = 0.2、pH值为7.00或7.50的条件下，于6 - 30摄氏度的温度范围内获得。所有反应均为吸热反应，且ΔH值随磷酸链长度和温度的增加而增大。将这些ΔH值与先前获得的腺嘌呤核苷酸 - 镁配合物的ΔH值进行了比较。对量热法和电位法测得的ΔH值进行了比较。在低温下观察到的这些结果之间的差异使我们推测锰离子的存在会诱导核苷酸聚集体的形成。该假设通过差示紫外光谱得到了证实。
