哺乳动物基因组中含有高比例的与核糖体蛋白L19相对应的加工假基因。

The mammalian genome contains a high proportion of processed pseudogenes corresponding to ribosomal protein L19.

作者信息

Shiran A, Flusser G, Aloni R, Meyuhas O

机构信息

Department of Developmental Biochemistry, Hebrew University, Hadassah Medical School, Jerusalem, Israel.

出版信息

Biochem Int. 1990 Dec;22(5):921-8.

PMID:2099152

Abstract

The mammalian genome contains multiple copies of ribosomal protein (rp) L19-related sequences. Screening of mouse and rat genomic libraries with cloned rpL19 cDNA yielded seventeen independent lambda Charon 4A recombinant phages containing twelve and five genes for mouse and rat rpL19, respectively. Structural analysis indicated that all of these rpL19 genes contain the entire coding sequence (approx. 700 bp) but lack introns. The nucleotide sequence of a mouse gene (rpL19-17), exhibiting the highest homology with the mouse rpL19 cDNA, revealed genetic lesions which would preclude the translation of an intact protein, from a putative transcript. Based on these features we propose that these clones represent processed genes of which most, if not all, are pseudogenes.

摘要

哺乳动物基因组包含多个核糖体蛋白（rp）L19相关序列的拷贝。用克隆的rpL19 cDNA筛选小鼠和大鼠基因组文库，分别得到了17个独立的λ噬菌体Charon 4A重组噬菌体，其中包含12个和5个小鼠和大鼠rpL19基因。结构分析表明，所有这些rpL19基因都包含完整的编码序列（约700 bp），但没有内含子。与小鼠rpL19 cDNA具有最高同源性的一个小鼠基因（rpL19 - 17）的核苷酸序列显示，从一个假定的转录本来看，存在一些遗传损伤，这些损伤会阻止完整蛋白质的翻译。基于这些特征，我们提出这些克隆代表了加工基因，其中大多数（如果不是全部的话）是假基因。