Kokeguchi S, Kato K, Kurihara H, Nishimura F, Murayama Y
Okayama University Dental School, Japan.
Dent Jpn (Tokyo). 1990;27(1):29-34.
Outer membrane proteins (OMPs) were extracted from whole cells of several Porphyromonas and Prevotella strains and their OMPs profiles were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The SDS-PAGE analysis revealed that OMP profiles of Porphyromonas and Prevotella strains show species-specific patterns and P. gingivalis characteristically had two kinds of major outer membrane proteins (MOMPs). A 53 Kd MOMP from P. gingivalis FDC 381 and a 67 Kd MOMP from ATCC 33277 were purified. Sera from periodontitis patients and healthy subjects were analyzed for immunoreactivities against both the purified MOMPs of P. gingivalis by immunoblotting analysis. The sera from 18 patients reacted to the 53 Kd MOMP, 10 to the 67 Kd MOMP, and only three sera reacted to both MOMPs. The sera of healthy subjects also reacted, but weakly, to either the 53 Kd or 67 Kd MOMP. The SDS-PAGE OMP profiles prepared from 13 clinical isolates of P. gingivalis and immunoblotting analysis of human sera against the two kinds of P. gingivalis MOMPs indicate that periodontal diseases resulting from P. gingivalis are initiated and sustained by at least two MOMPs of P. gingivalis.
从几种卟啉单胞菌属和普雷沃菌属菌株的全细胞中提取外膜蛋白（OMPs），并用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）检测其OMPs图谱。SDS-PAGE分析显示，卟啉单胞菌属和普雷沃菌属菌株的OMP图谱呈现出种属特异性模式，牙龈卟啉单胞菌典型地具有两种主要外膜蛋白（MOMPs）。纯化了牙龈卟啉单胞菌FDC 381的一种53 Kd MOMP和ATCC 33277的一种67 Kd MOMP。通过免疫印迹分析，检测了牙周炎患者和健康受试者血清对牙龈卟啉单胞菌这两种纯化MOMPs的免疫反应性。18例患者的血清与53 Kd MOMP发生反应，10例与67 Kd MOMP发生反应，只有3例血清对两种MOMPs都有反应。健康受试者的血清也有反应，但较弱，对53 Kd或67 Kd MOMP其中之一有反应。从13株牙龈卟啉单胞菌临床分离株制备的SDS-PAGE OMP图谱以及人血清对牙龈卟啉单胞菌两种MOMPs的免疫印迹分析表明，由牙龈卟啉单胞菌引起的牙周疾病是由牙龈卟啉单胞菌的至少两种MOMPs引发并持续的。
