Gómez Dumm C L, Souto M, Nash R E, Echave Llanos J M
Chronobiologia. 1978 Apr-Jun;5(2):127-36.
An electronmicroscopic study of STH cells from mice bearing transplanted hepatomas was performed at different time points, in a circadian period. Normal mice served as controls. The STH cells of control animals showed circadian variations inplasmic reticulum and lysosomes, which suggest increased secretion of growth hormone along the light peroid. In mice bearing hepatomas the morphologic aspect of these organelles would also indicate an increase of STH elaboration at 1200 and 1600. These changes were more remarkable than in controls and mainly consisted of hypertrophy of the Golgi complex, extended and compact endoplasmic reticulum and presence of large amounts of lysosomes. Besides, another peak of secretion seems to be present at 0000, considering the dilatation of the Golgi complex and endoplasmic reticulum cisternae. Some findings are coincident with observations made by others in the hypophysis of animals bearing transplantable tumors, where circadian periodicity was not studied. The STH cytological circadian variations could be correlated with other variations could be correlated with other variables, such as STH values in plasma and hypophysis, and DNA synthesis and mitotic activity of SS1-H hepatoma.
在一个昼夜周期的不同时间点，对移植了肝癌的小鼠的促生长激素细胞进行了电子显微镜研究。正常小鼠作为对照。对照动物的促生长激素细胞在粗面内质网和溶酶体方面表现出昼夜变化，这表明在光照期间生长激素的分泌增加。在患有肝癌的小鼠中，这些细胞器的形态学方面也表明在1200和1600时促生长激素的分泌增加。这些变化比对照组更显著，主要表现为高尔基体复合体肥大、内质网扩张且紧密以及存在大量溶酶体。此外，考虑到高尔基体复合体和内质网池的扩张，似乎在0000时存在另一个分泌高峰。一些发现与其他人在患有可移植肿瘤的动物垂体中所做的观察结果一致，在那里没有研究昼夜周期性。促生长激素细胞的细胞学昼夜变化可能与其他变化相关，例如血浆和垂体中的促生长激素值，以及SS1 - H肝癌的DNA合成和有丝分裂活性。
