Wells J A, Cunningham B C
Department of Biomolecular Chemistry, Genentech, Inc., South San Francisco, CA 94080.
Biochem Soc Symp. 1990;57:143-5.
Mutagenic analysis are described for probing and designing binding determinants of two different proteins. The first protein is an enzyme, subtilisin, for which structural models were available for the enzyme-substrate complex. The second protein is human growth hormone for which a structural model of its complex with receptor was not available. In both cases, it was possible to identify and engineer binding determinants that lead to large changes in affinity.
描述了用于探测和设计两种不同蛋白质结合决定簇的诱变分析。第一种蛋白质是一种酶，枯草杆菌蛋白酶，其酶 - 底物复合物的结构模型是可获得的。第二种蛋白质是人生长激素，其与受体复合物的结构模型不可获得。在这两种情况下，都有可能识别和改造导致亲和力发生巨大变化的结合决定簇。
