Márquez M, Silva M, Pérez-Bendito D
Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
J Pharm Biomed Anal. 1990;8(7):563-7. doi: 10.1016/0731-7085(90)80080-9.
An automatic kinetic method for the determination of micro amounts of vitamin B12 in pharmaceutical samples based on the fast formation of a coloured complex between the cobalt contained by this vitamin and PAR [4-(2-pyridilazo)resorcinol] in a weakly alkaline medium and on the use of the continuous addition of reagent technique for the mixing of sample and reagent is reported. The reaction is monitored by measuring the changes in the absorbance at 510 nm characteristic of the complex formed. The linear range of the determination is 1.1-34.5 micrograms ml-1 and the relative standard deviation is 1.2%. The sample throughput is 75 h-1 (triplicate runs). The results obtained in analyses of pharmaceutical samples showed excellent correlation with nominal contents and the results of atomic absorption spectrophotometric analyses.
报道了一种自动动力学方法，用于测定药物样品中的微量维生素B12。该方法基于在弱碱性介质中，维生素所含的钴与PAR[4-(2-吡啶偶氮)间苯二酚]快速形成有色络合物，并采用连续添加试剂技术来混合样品和试剂。通过测量在510nm处形成的络合物的特征吸光度变化来监测反应。测定的线性范围为1.1 - 34.5微克/毫升，相对标准偏差为1.2%。样品通量为75次/小时（三次重复测定）。药物样品分析所得结果与标称含量以及原子吸收分光光度分析结果具有极好的相关性。
