Urbanik T, Russa R
Institute of Microbiology, M. Curie-Skłodowska University, Lublin, Poland.
Acta Biochim Pol. 1990;37(4):417-31.
The structure of the lipid A from S. typhimurium harboring the derepressed plasmids Col Ib is very similar: i, 1,4'-bis-phosphorylated-beta-1',6-linked glucosamine disaccharide forms a backbone of the lipid; ii, lipid preparations contain four residues of 3-hydroxytetradecanoic acid at positions C3, C3' and the amide linked at C2, C2' and two free hydroxyl groups at positions C4 and C6'. Differences concern: i, substitution of phosphoryl groups by 4-amino-4-deoxy-L-arabinopyranose and phosphorylethanolamine in S. typhimurium with Col Ib plasmids; ii, the degree of acylation of hydroxyl groups of 3-hydroxytetradecanoic acid by myristic, lauric and palmatic acids; iii, presence of tridecanoic acid bound to hydroxyl of 3-hydroxy-tetradecanate residue in S. typhimurium with Col Ibdrd2 plasmid. Lipopolysaccharides from the plasmid mutant strains express several times higher lethal toxicity in chick embryos compared to lipopolysaccharides from the strain with the wild type Col Ib.
携带去阻遏质粒Col Ib的鼠伤寒沙门氏菌的脂质A结构非常相似：i，1,4'-双磷酸化-β-1',6-连接的葡糖胺二糖构成脂质的主链；ii，脂质制剂在C3、C3'位置含有四个3-羟基十四烷酸残基，在C2、C2'位置有酰胺连接，在C4和C6'位置有两个游离羟基。不同之处在于：i，携带Col Ib质粒的鼠伤寒沙门氏菌中4-氨基-4-脱氧-L-阿拉伯吡喃糖和磷酸乙醇胺取代了磷酰基；ii，3-羟基十四烷酸羟基被肉豆蔻酸、月桂酸和棕榈酸酰化的程度；iii，携带Col Ibdrd2质粒的鼠伤寒沙门氏菌中十三烷酸与3-羟基十四烷酸残基的羟基结合。与具有野生型Col Ib的菌株的脂多糖相比，质粒突变株的脂多糖在鸡胚中表现出高几倍的致死毒性。
