猪主动脉和血清中甘油酯水解酶的底物特异性。

The substrate specificity of glycerol ester hydrolase from pig aorta and serum.

作者信息

Pioruńska-Stolzmann M

机构信息

Department of General Chemistry, Medical Academy, Poznań, Poland.

出版信息

Acta Biochim Pol. 1990;37(4):457-62.

PMID:2100898

Abstract

The activity of glycerol ester hydrolase (GEH) from aorta wall, at optimum pH and triacylglycerol substrate concentration (optimal for each substrate) decreased in the following order: C18:1 greater than or equal to C18:2 greater than or equal to C18:0 greater than or equal to C18:3 greater than or equal to C16:0. At optimum pH and the same substrate concentration (1 mM), the activity of GEH from aorta wall decreased in a slightly different order: C18:1 greater than or equal to C18:2 greater than or equal to C18:3 greater than C18:0 greater than C16:0 and that of the enzyme from serum in the order: C18:1 = C18:3 greater than C18:2 greater than or equal to C16:0 greater than or equal to C18:0. These differences in substrate affinity of GEH may influence the metabolism and accumulation of acylalcohols and alcohols in arterial wall and serum.

摘要

在最佳pH值和三酰甘油底物浓度（每种底物的最佳浓度）条件下，主动脉壁甘油酯水解酶（GEH）的活性按以下顺序降低：C18:1≥C18:2≥C18:0≥C18:3≥C16:0。在最佳pH值和相同底物浓度（1 mM）时，主动脉壁GEH的活性降低顺序略有不同：C18:1≥C18:2≥C18:3＞C18:0＞C16:0，而血清中该酶的活性顺序为：C18:1 = C18:3＞C18:2≥C16:0≥C18:0。GEH底物亲和力的这些差异可能会影响动脉壁和血清中酰醇和醇的代谢及积累。