Soares M F, Perini A, Mota I, Macedo M S
Centro de Formação e Pesquisa em Imunologia, Instituto Butantan, São Paulo, Brasil.
Braz J Med Biol Res. 1990;23(6-7):589-92.
In order to characterize the component(s) of Ascaris suum responsible for damping of the IgE antibody production we demonstrated that the extract incubated in sodium acetate buffer, pH 4.5, maintained its suppressive effect and the same protein banding pattern by SDS-PAGE. Elimination of the lipoprotein components of the extract also left its damping properties unchanged. SDS-PAGE of the lipoprotein-free extract revealed practically the same pattern as shown by the whole extract, except for the high molecular weight polypeptides. These results indicate that the suppressive component(s) of A. suum did not precipitate and retained their activity at low pH. In addition, they appear not to be lipoproteins.
为了鉴定猪蛔虫中负责抑制IgE抗体产生的成分，我们证明，在pH 4.5的醋酸钠缓冲液中孵育的提取物通过SDS-PAGE保持其抑制作用和相同的蛋白条带模式。去除提取物中的脂蛋白成分后，其抑制特性也未改变。无脂蛋白提取物的SDS-PAGE显示出与全提取物几乎相同的模式，除了高分子量多肽。这些结果表明，猪蛔虫的抑制成分不会沉淀，并在低pH下保持其活性。此外，它们似乎不是脂蛋白。
